Adsorption Quantification II

One question we recently had was what does it look like when we perform a separation experiment when a membrane has a pinhole. I selected two membranes off 621 that appeared to have pinholes on both slits. I applied 1:2 standards to the top and allowed to diffuse for 24 hours. I collected the retentate…

I talked about this yesterday in lab meeting, but here is the data.  Since the components of HBSS by themselves did not cause discoloration (HBSS components), I adjusted the concentrations of NaCl, KCl, glucose, Na2HPO4 and NaHCO3 to match that of DMEM.  The control was a wafer in DMEM/F12 with 10% FBS.  None of these…

Here is a summary of some of the talks I attended at BMES 2017, mainly from the Nano and Micro- technologies track. This is an ongoing post, I will post more comments, links, and figures. Anna Kalmykov (Tzahi Cohen-Karni) Carnegie Mellon University: 3D Nanosensors for Electrical Interrogations of Engineered Micro-tissues. Overall synthesis of nanostructures to be used…

We will devote the first NRG meeting of each month to journal club. First up is Karl who will review two papers describing the use of single nanopore membranes as a method of characterizing particle size and zeta potential on a particle by particle basis. The technology produces intriguing data sets like the one shown…

I’ve been trying for some time to make chips that can dynamically change their surface charge. To this end I’ve made the following NFT chips, which have 12 nm of silver coated in 10 nm alumina:   Note the Aluminum foil coating the insides of the gel box. The silver layer is either photoactive or…

Christine and I have been using the present Corning 24-well plate definition in the Tecan for our experiments so far.  Yesterday I placed a pnc-Si chip in the (approximate) middle on the bottom of 1 of the 24-wells.  I did a 500nm absorbance scan in a circular pattern (15×15 points) – to get data similar…