Adsorption Quantification II
I’ve updated the figure from this post to add new data from oxidized and pre-washed glass samples. We now have possession of teflon material, which I will be testing this week.
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Wafers 620/621
Today I etched wafer 621, which is the pair to 620. While w620 has a relatively low pinhole density, w621 shows a much higher occurence of defects. Since the front-end processing and thermal treatment was identical for these wafers, I believe that the variability lies in the etch process. Looking at the two wafers side-by-side,…
Membrane Discoloration
15um beads on discolored membrane surface, having some slits where the beads are not found The localization of microbeads suggest that they have fallen across the slits in the membrane Transparent membrane with microbeads on its surface These membranes are not treated with cell media, hence are not discolored. There’s a slit which appears to…
Woods Hole Workshop on Microplastics in the Oceans
Summary: Dan and I went to Woods Hole Oceanographic Institute to learn about the current state of microplastics research. There were a number of things that we learned that we did not know. The common sentiment among researchers agreed climate change was a much bigger threat to humans than microplastics, but the reality and immediacy…
Record porosity and size in 15 nm pnc-Si
As a continuation of the substrate bias series, I deposited a 15 nm film with 05 W substrate bias and annealed at 1000 C. TEM shows that this condition generates a 13.4% porosity and a max pore size of ~40 nm! Here is a comparison of all the bias conditions tested thus far: I believe…
Flow Cell/µSiM Testing – Push versus Pull Flow Modes
Introduction Static endothelial cell cultures have been routinely performed and optimized on the µSiM platform for multiple membrane and cell types. In order to increase the physiological relevance of our device with tissue vasculature mimetics, we need to incorporate fluid flow to emulate shear stresses experienced by endothelium under physiological circulatory conditions. Recently Mehran has…
WEKA-Based Pore Counting Analysis of TEM Images
Summary There’s a lot to be desired in counting pores in grayscale images, especially when the contrast differences are low. A human eye is usually much better at picking out slight differences in contrast, and this can mean the difference between an open and closed pore. Differences of 5 nm can be significant in certain…