Makerware 3d print Preview
One neat feature of the Makerware software is to visualize the printed layers before actually printing them. You can catch a lot of errors with this feature; I highly recommend it.
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Culture divider insert assembly and test
Introduction We are designing a cell culture chamber insert to allow side-by-side co-culture of cells and ultimately triculture in the µSiM devices. Materials and Methods ALine devices assembly according to the “ALine Modular Device Assembly” instructions. 2-slot NPN membranes applied (NPSN100-2L, nanoporous silicon nitride 100 nm thick, 2 windows). Straight tweezers and chip tweezers are…
BBB Device 2.7 Fabrication with Mortar Bonding
Fabricate ITO Electrodes Deposit 2500 Angstroms, ITO, Room Temperature Using Kurt Lesker Sputterer (Use ITO Variable Recipe, takes about 1.5hr) Tape in Microscope Slides and Coverglass into Machined Shadow Mask using Kapton Tape Anneal in Toaster Oven, 450 Fahrenheit, 1hr Drops resistance from > 40 Megaohm to < 5 kohm. Measure from edge of Pad…
Rotation Project Complete: Dialysis of cytochrome c across a commercial membrane
Dan Steiner Rotation Summary Abstract The purpose of this rotation project was to develop and test a clampable system for eventual use in scalable nanomembrane dialysis systems. The nanomembrane dialysis system will be miniaturized into a compact, wearable hemodialysis device that will provide more effective human dialysis treatment (Johnson et al. 2013). For initial tests, a…
New Alt. Membrane Release Recipe (Nitride RIE)
I have lost many chips during my MgF2 or Au or Pt process due to yield problems with my RIE. I have wondered if the problem was due to power spikes. I have observed that my most poorly yielding etch runs usually have uneven power or power spikes during the run. Increasing window size (active area)…
Cleaning MgF2 Nanomembranes after International Shipping
I had previously manufactured some freestanding MgF2 nanomembranes (50 nm MgF2, starting from Wafer Template 1174) for my work here in Nottingham. I shipped about 75 chips at the beginning of July in my 3d printed holders, however, they arrived with a noticeable film discoloration (tending to blue/tan, rather than the nice purple color I had…
Wetting of Nanocavities using Isobaric Cooling
Summary and Instructional video The simplest way to get something wet is to allow water to condense on the surface of the something. In our case, we have a number of nanocavities (~ 0.1 femtoliters) with porous tents over them. The easiest thing to do is simply chill the substrates down to allow condensation to form….