Makerware 3d print Preview
One neat feature of the Makerware software is to visualize the printed layers before actually printing them. You can catch a lot of errors with this feature; I highly recommend it.
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YAP/TAZ Staining on Membranes and Nonporous SiO2
As we’re uncovering more bits and pieces of data, we kept being lead to the idea that our membranes may be made out of SiO2 and have a modulus in the gigapascal range, but cells seem to perceive them as soft substrates. After looking at focal adhesions, fibronectin deposition, and cell/cell junction formation, we’re still sticking…
Nanomembranes+Microfluidic PETLs Integration
Our collaborator Fernando has made a really nifty way to make microfluidics work with lamination pouches. These flat pouches can be sealed together using a heat activated glue, which can be done consistently using a 15 for a 100-pack of 8.5″x12″ pouches + $40 laminator Put multiple materials and multiple chips in the same microfluidic package….
Bonding PDMS to PET films
PET is a very common material used in various cell culture applications. Unfortunately it’s chemical inertness makes it immune to plasma activation, and we can’t bond PDMS devices to PET. I am working with a PET films coated with Indium Tin Oxide (ITO) on one side of the film. The film, in terms of texture,…
Wetting of Nanocavities using Isobaric Cooling
Summary and Instructional video The simplest way to get something wet is to allow water to condense on the surface of the something. In our case, we have a number of nanocavities (~ 0.1 femtoliters) with porous tents over them. The easiest thing to do is simply chill the substrates down to allow condensation to form….
Permeance tests with new Omega rotameter
Yaron has acquired an Omega rotameter (FL-3803ST) with interchangeable flow tubes. The tubes operate in three different ranges low (6-61 mL/min, 042-15-N), medium (150-1,682 mL/min, 082-03-N), and High (1,000-23,752 mL/min, 034-39-N). I’ve attached an excel file with the calibration data plotted. We fit the curves with polynomial trendline to get equations. Also in the workbook…
Small Animal Model Hemodialysis Set-Up
Sprague-Dawley rats will be purchased from Harlan. These pre-manipulated animals will have been catheterized prior to shipment. We will feed them an adenine rich diet to induce uremia, high levels of urea in the blood. Once uremic, a rat will be connected via blunted needles to the dialysis system. Heparin, or other substances, may be…