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Lately, Anant and I have been doing our discoloration experiments under purposely harsh conditions so we don’t have to wait for multiple days for experiments to finish. We have lots of evidence that the 3D format slows down discoloration (post1, post2, post3). This experiment was to test how long samples will last in 3D format…

My PhD exam was postponed (tentatively to early September but it’s up in the air still) due to an impending bureaucratic nightmare, so for the last few weeks I have been running more filter experiments, and I have discovered some interesting trends. While it is generally the case in nanopore experiments that the average dwell…

Background:  The purpose of these experiments was to investigate the formation of gap junctions between co-cultured HUVEC and ADSC across 0.5µm HP SiO2 and 0.4 µm HP Track-Etched membranes. Gap junctions are channels that allow small molecules to pass directly from cell to cell. Electron micrographs show that the membranes of two adjacent cells are…

There is strong evidence that the recent pinhole breakout is due to copper contamination in our process. I have attached two image – An SEM showing a cuprous creature eating one of our membranes and an EDX (Energy Dispersive X-ray?) spectra identifying it as a copper-rich silicide. This would also explain Dave’s TEM image of…

This is just a little update on the results of the pnc-Si, cellulose, and pes separations.  When I added up the retentate and filtrate, it didn’t always equal the intensity of the starting sample (keeping in mind that I was unable to collect filtrate from PES and should re-try that separation). I took the percent…

The SiO2 coated samples (from W335 & W341) were found completely discolored after 5 days in DMEM (benchtop). Thus, silicon dioxide coating didn’t prevent discoloration as much as the RTP treatment. There’s a need to check the discoloration profile of the SiO2 coated samples within 5 days. Till 24 hrs. there was no discoloration observed….