Journal Club: Preview from Sabrina Leslie – CLiC with flow

Just to double check the numbers I calculated on Tuesday I ran some wet-wet trials with 100K and 300K Nanoseps. Based on the wet-dry results we would expect that the permeability for the 100K Nanosep would be higher than that of the 100K Microcon. It goes without saying that the 300K Nanosep would also be…

For year one of the Trace grant, I will be focusing on the development of a standard operating procedure for the uSiM-CA. To begin this process, I am transferring all of my current operating procedures from the Andor dragonfly confocal scope to the new Etaluma microscope. The Etaluma scope will be automated, collecting images of…

In this post I will describe recent streaming potential measurements for PES membranes and the calculated apparent zeta potentials from these measurements. Setup: 1. Fabricate Ag/AgCl electrodes: Silver wire at the anode in 1M KCl at 1mA current for 20 minutes. 2. Assemble streaming potential device: Electrodes are inserted into silicone chord o-rings and chamber…

Previously I had posted a chart depicting the permeabilities of SepCon membranes from w310. I went back and noticed some errors in the equations. Here is an updated version of that chart. Also included is a sample where I used the ultracentrifuge to clarify the protein right before the experiment, which should remove any larger…

Introduction In response to recent requests by our reviewers, I am looking to address two main concerns: 1) How does a collagen gel backing affect leukocyte transmigration, and 2) How does shear influence leukocyte transmigration. While we believe these areas are rich in information and need a significant amount of focus to tackle, we do…