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Barnstead and Hopeman water OK
I did two tests using water straight out of the Barnstead and with water from the cleanroom in Hopeman. I was able to maintain high permeability using the same membrane for at least three successive trials with both. It looks like the culprit is the Nalgene container used to store the water. For any sensitive…
Young's modulus and residual stress of pnc-Si deposited with different substrate biases
Last week, I reported on the the morphology of pnc-Si deposited with different substrate biases. We hypothesized that the reason for this was due to the different initial film stress of the as-deposited a-Si. Unfortunately, the pressure-displacement method does not allow us to measure amorphous films since it requires the membrane to be nearly flat…
Defining “Resolution” in our Sieving Model
In my earlier posts, as well as in Josh’s NPN paper and Tom’s ACS nano paper, we’ve talked a lot about the resolutions of nanoparticle separations. What we mean by it is how well the membrane is able to discriminate between two similarly sized particles. For instance, we predict that a series of gold nanoparticle filtrations…
"Phantom" Curves on the TECAN Mess with Reproducibility and Accuracy
At some point, when things settle down, I’ll create a page dedicated to the TECAN machine and document all of the troubleshooting and tricks I’ve learned so far. Before that happens I have another mystery to solve. Take a look at the following three scans: This first are some scans of BSA filtrates from…
Astrocytes on PET and pnc-Si transwells (1)
This is my first attempt to grow astrocytes (NG108-15 cell line) on PET and pnc-Si transwells. These are passage 8, seeded at 50000 cells/cm2 and grown for 7 days. Of the 8 pnc-Si transwells (RTP’ed SC104) I made, all but one survived this 7-day cell culture experiment. These cells were plated on the bottom on…
PDMS cell channel repeat
This is an update to my last post, where HUVECs mixed with collagen inside PDMS channels seemed to stay rounded up and not really do much of anything exciting. This time around I made a few adjustments: New collagen protocol – gives 1.5 mg/mL solution before mixing with cells: -10% 10x PBS -1.4% NaOH -38.6%…