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Jirachi has been working to make thin nafion films using our membranes as a support structure. Nafion is polymer that contains a sulfonate group, and as a result acts as a proton wire, allowing for cations (+) to be transported across a membrane, but not anions or electrons. Such a membrane can be used for filtering a…

The last time I did live imaging on the confocal microscope was in December of 2019. Then I was imaging the capture of 1.24 µm diameter beads in 1 µm slit pore membranes to visualize the difference between steric and affinity based capture. This time around, all of the materials are different, but the process…

Scope: Human umbilical vein endothelial cells (HUVEC) and adipose derived stem cells (ADSC) were co-cultured on SiO2 low density 3um pore membranes. The ADSCs were initially seeded onto the membranes, at 6 hours the membranes were flipped over and placed into a 24 well plate and HUVECs were seeded. Cells were grown for 3 days…

Bernhard Group: Discoloration was observed during DNA separation experiments.  Further study led to these results: No discoloration observed with low pH buffered NaCl. Discoloration with high pH buffered NaCl. Worried that Cl was the culprit, sodium acetate was tested.  Discoloration observed with sodium acetate. In one early experiment, 4 areas were tested on one chip. …

Here is a look at all our water permeability through nanomembranes to-date. There appears to be subtle trends showing increasing permeability with radial position (r – values) and a not-so-subtle trend showing decreasing flow with protein (cyt and albumin). Note that with the more recent membranes we’ve been able to do repeats. The permeabilities are…

I tested five types of track-etched membranes (with pore diameters 0.1, 0.08, 0.05, 0.03 and 0.01micron). All of them are 6micron thick and usually have 14-16% porosity. First experiment was to confirm the official air flow rates data with our setup. The measurements are taken at 10psi (as the ones that company did). The results…