Webb Visit Slides

There has been some confusion lately with what we should name the compartments in diffusion.  Here’s what I’ve come up with: Top/Compartment with initial sample: Retentate Bottom/Compartment to which things diffuse: Dialysate The original word we were using for dialysate, filtrate, implies an active process and was criticized by scientists at NAMS.  While dialysate implies…

I’ve been thinking of demos/experiments to do with my upcoming class and one of them is SEM.  I was thinking of handing out some unknown nanoparticle solutions and have them figure out the size and maybe material by SEM, AFM and/or DLS. I’m approved to run the SEM now, so I hopped on today to…

Yesterday I posted the results from an experiment testing diffusion of BSA and DDM with salts (10mM) across “treated” and “untreated” SC256 samples. The results posted yesterday showed relatively equal amounts of DDM diffusion in both the “treated” and “untreated” case. For the BSA diffusion however the results showed that a greater amount of BSA…

Demos of the Molecular Devices M2e and Tecan Infinite M200 are done. Erik from Tecan left us the M200 to use until October 6th.  Please come try out your assays on it.  Or check sensitivities.  Jess or I could probably steer you in the right direction as far as the software is concerned. My impressions…

First, a quick publication update. Recently we’ve published a paper on our efforts towards computationally analyzing polymorphonuclear leukocyte (PMN) trafficking on a vascular endothelium via hand-built µSiM flow cell devices. We record videos in phase contrast with a 40x objective and are able to delineate PMN state, speed, and persistence by leveraging ML algorithms and…

Permeability was calculated with Dagan theory for membranes in this post.  Pore processing was performed and r.mat files were run through the programs used to calculated permeability in submitted Science paper. No Carbonization Experimental: 591 ul/(min*PSI*cm^2) Theoretical: 801 ul/(min*PSI*cm^2) Carbonized (6.2 nm taken out of diameters) Experimental: 289 ul/(min*PSI*cm^2) Theoretical: 438 ul/(min*PSI*cm^2) It seems that there…