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10 dynes/cm^2 bottom channel Aline
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AFM Measurements of Glycocalyx in the Upside Down µSiM
Slide deck from Large Group Meeting 12-21-12 (PPT) Access requirements for the AFM cantilver to contact the cells requires the cells to be grown on the membrane surface in what is typically the bottom channel of A-line devices (Typical A-line configuration is shown in slide 2). A PDMS block is attached to the well side…
A Power-free Pneumatic System for Controlling Fluid Flow
ByMeng HsuIntroduction When searching for a flow control system for microfluidic cell culture, we look to balance the ease of use, accessibility, and precision of fluidic control. For instance, researchers have explored different powered actuation methods for miniaturized precise proportional fluid control. These actuation methods include electromechanical, electrostatic, electromagnetic, piezoelectric, and thermal-based. The necessary power sources,…
Monocyte Transmigration in μSiM Devices – Optimizing Microporosity for Future Studies
Introduction Infection or injury of tissue results in inflammatory signalling that motivates leukocyte recruitment to an inflamed location. Monocytes in particular are implicated in both pro inflammatory signalling through the M1 macrophage phenotype and regulation through the M2 macrophage phenotype. The μSiM devices developed in our lab have yet to assess/optimize monocyte transmigration from the…
hiPSC Differentiation into Brain Pericyte-like Cells, Round 2
Introduction Breakdown of the blood-brain barrier (BBB) is one of the earliest signs of sepsis and is linked with long-term cognitive impairment. As animal models poorly recapitulate human sepsis, alternative approaches are direly needed to understand sepsis-associated brain injury and develop strategies that protect against cognitive decline. To fill this gap, we are developing a…
COMSOL simulation on sampling method to measure membrane permeability
Background We at Michigan have been aiming to perform sequential small volume sampling coupled with our ultrasensitive digital immunoassay to provide longitudinal data from the uSIM. We have encountered repeatability problems as well as low molecule abundancy with shortly incubated samples collected from the bottom microfluidic channel. Therefore, COMSOL simulation work has been done to…