Jim’s Talk at Spring Brain Conference in Sedona, AZ

Here is the analysis of the wafers used in the pressurized gold separations: SC 199   Stat Result Mean Equiv Diameter 17.84 nm Median Diameter 18.30 nm Porosity 2.2% Weighted Mean Diameter 20.81 nm 90% Diameter 26.64 nm Roundness .782 Separates 10 nm from 15 nm w225   Stat Result Mean Equiv Diameter 18.33 nm…

Recently, SiMPore has been working on a new chip design which involves incomplete etches on both sides in order to create a fluidic system built directly into the silicon, consisting of many long, shallow trenches. These trench chips are designed to obviate the need for silicone gaskets on one side of the chip, reducing the…

I am trying to grow endothelial cells under flow and measure electrical resistance of the culture during the flow-based growth. I am using a peristaltic pump to generate the flow. I am using microporous membrane with 3 micron pores with 3 micron spacing, and 20% porosity for this experiment. Microporous membranes can facilitate neutrophil transmigration,…

Previously we have studied protein adsorption to pnc-Si membranes by TEM and fluorescence. Neither of these methods will work with polymer membranes. Polymer membranes are too thick to view using TEM. Polymer membranes also autofluoresce creating a lot of noise while using our fluorescence method. Here I outline a new method of studying adsorption to…

We have two cutoffs for the Millipore Biomax PES membranes: 50kD and 30kD.  I tested the “EO” rates for both of these membranes and plotted them in this figure with standard deviations (3 trials  of 50kD and 4 of 30kD). 50kD has a significantly higher rate of “electro-osmosis.”  I will try to compare these rates…