Silicon Ideal to Culture Patient-On-a-Chip | Insight & Intelligence™ | GEN

Previously, I had gotten some inexplicable results showing that increasing salt concentration decreases the flow through a membrane. This makes no sense (and goes against our previous data) because we would expect electrostatic forces to counteract the flow, an effect called counterelectroosmosis by Zydney: “The situation in the presence of a net fluid flow through…

I used the source meter that Chris brought over and tested the voltage at points across the chamber. I set up the system with 1x PBS at 15V (~10mA) with a pinholed membrane. I tested the voltage drop at the outer edges of the well, in the center, and the inner edge of the chamber…

UPDATE: A look at long and short crystal dimensions (9/1/10): DLS vs. Crystal Sizes – Newest version 8/30/10 Here is a comparison of sieving curves using DLS or estimated crystal dimensions of proteins. DLS: 24 hours sieving curve. 48 hours sieving curve. Estimated longest crystal dimension: 24 hours sieving curve. 48 hours sieving curve.

I guess we weren’t sure if I was actually drawing rhodamine back through the membrane or just bleaching it. So I made a new apparatus which consisted of a sawed off sepcon mounted on a slide with channels etched into the lower plastic of the device to connect a pbs resevoir on the slide to…

We have often encountered cytochome C dimers as part of our work. For example in this figure from Jess that we often refer to in our hemodialysis work. It turns out that Cyto C polymerization is a well known phenomenon. The dimers are very tough and survive the denaturating conditions in Jess’ gel. Here is…

One major problem that we faced with our current shear-free chemotaxis system is the lack of high image quality.  Since the silicon-nitride (SiN )grid is 100 um away from the coverslip surface on which the cells migrate, the out-of-focused SiN grid blurs the image.  This makes cell tracking much harder. An example of a movie…