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I’m having trouble getting the PDMS to cure on the NC. When I first started these experiments in August, the PDMS seemed to cure on the NC without a problem. Now, when I remove the PDMS + NC from the oven, after heating for 2+ hours at the highest setting, I notice that the PDMS…
Another one of the questions I’ve been trying to answer with regard to endothelial cell vacuoles is whether membrane mechanics or permeability drive vacuole expression. I’ve thought of several experimental designs to test this that haven’t worked (i.e., using very small volumes on the apical side to minimize permeability, using PDMS plugs to block the…
Today, I ran a quick experiment to confirm that water transport was not negatively affected by an aluminum oxide coating. I deposited 1.5 nm of Al2O3 onto a pnc-Si membrane which I then treated with an oxygen plasma in the YES system. I was curious to see whether the alumina coating along with O2 treatment…
Just wanted to show the detail of PETL channels. A couple of interesting things: The adhesive layer(s) are pretty thick, and look pretty smooth after heat curation. Also, the channels are slightly beveled, probably due to craft cutter blade.
UPDATE: Figure normalized by frontal surface area. This brings the glass data in line with pnc-Si (which is similar to my more recent fluorescence data). Here is my first try at putting together all the recent adsorption gels I’ve done. This data has all been obtained using the more vigorous washing step. All densitometry performed…
I followed Greg’s example and embedded a broken-membrane chip in a PETL. The idea was to use it as a negative control before I use an intact chip. Laminar flow should be maintained in this scenario in order for us to conclude that the membrane is responsible for mixing, should it occur. 4 layers…