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Microporous MgF2 summary
I’ve been gathering data for a paper using my microporous MgF2 nanomembranes so here are some sample figures for such a paper. Herein, we are trying to create a porous, Raman-compatible substrate for use in cell culture. Researchers have used Raman microspectroscopy to perform quantitative non-invasive measurements of biomolecules in cells. It’s a very exciting…
COMSOL Model Files Download Page
This post is not an update on my research. I am creating this page to house download links to the COMSOL Multiphysics models I’ve constructed in the course of my work here in the McGrath Lab. This page is linked to in my thesis for legacy purposes. Important: these files have been uploaded with the…
A nifty little Matlab code to show why we need to wet the backsides of the membranes
I couldn’t really make sense of what was happening in this post, and in particular this figure threw me for a loop: So to help myself understand the relationship I whipped up a little matlab app to show the relationship a little clearer: Moving the sliding bar on the bottom changes the y-position…
Process Flows
What process flows have I used to create my devices? Standard Cut out gaskets (10 min) Embed Electrode (face down) (3 hrs) Ozone Bottom, Interstitial, Filter and Top channels together (1 hrs, cure overnight) Ozone Bottom Electrode (1 hr, cure overnight) Ozone Top Electrode + Feet (1 hr, cure overnight) Weaknesses are that there are…
Rotation Project: The Influence of Collagen Gels and Nanoporous Chips on Shear Based Endothelial Cell Alignment
ByAlecAs an introduction, my name is Alec Salminen. I received my BS in Biomedical Engineering at the University of Arkansas and am currently in my first year of the BME PhD program at the University of Rochester. As my rotation in the McGrath lab gets underway, I would like to share with the group my…
hiPSC Differentiation to Endothelial Cell Culture Method, Brain Microvascular Endothelial Cells (EECM-BMEC-like cells), Round 2
Introduction This post highlights results from my second attempt to differentiate hiPSCs into endothelial progenitor cells EECM-BMEC-like cells in our lab. For details on methodology, see Nishihara et al., FASEB, 2020 and my previous post. Methods Cells were seeded at 70,000 and 100,000 cells/well on D-3. Only the 100,000 cell/well plate was sorted. Due to media being backordered,…

