I measured burst pressures on two non-unifrom wafers w391, w393. They have lots of pinholes (especially w391) but the pressure they withstand is pretty high. I expected it to be much lower after looking at the membranes in the microscope.
Here are some high resolution TEM images from a SiMPore-UIUC collaborator. Chris says: The high resolution stuff looks pretty cool, especially the zoom-in on the 8nm pore. I the most obvious crystal planes are <111> with 0.314 nm spacing. If you look carefully, some 0.192 nm <110> planes and 0.136 nm <100> planes can also…
Two experiments were performed to explore application of SiMPore filters within INT’s microfluidic cartridges to allow the capability for pathogen type specificity in nucleic acid purification. 1) The first experiment tested the efficiency at which nucleic acids could pass through the membranes. T4 polynucleotide kinase was used to 32-P radiolabel two different sized PCR products:…
I’ve tested several membrane types with the streaming potential apparatus in order to calculate zeta potential. Here are the results: All materials in the above chart have negative zeta potentials. Pnc-Si seems to have the highest overall potential, followed by PES then cellulose. Cellulose is prone to cracking and bursting at pressure above 7 PSI….
I’ve suspected that the barrier function of endothelial cells might be surface-dependent (that is, cells grown on the pnc-Si side vs. cells grown on the well side). I hypothesized that the interface between membrane and well walls would alter the continuity of the endothelial monolayer when cells are grown on the well wide. To test…
As part of the ongoing study of NPN membrane filtration characteristics I used the SEM to look at membranes after they were used. Unless otherwise indicated, files with a “t” (top) indicate the chip was membrane side up. “b” stands for the bottom, or trench side. In a standard forward centrifuge setup we expect the…
Introduction: Developing innovative therapeutics and treatment approaches with improved effectiveness and safety necessitates the use of cell co-culture models and barrier environments that mimic in vivo conditions. These models involve culturing two or more cell types together in vitro, allowing researchers to study cellular interactions, signaling pathways, and responses that closely resemble those seen in…