Rhodamine and Fluoroscein Diffuse through SC269
Yesterday I posted my results from a BSA diffusion experiment using “treated” and “untreated” SC269 samples. To ensure that the pores in SC269 are indeed open I took a step backwards and set up a diffusion experiment using small dye molecues; rhodamine and fluoroscein. Using Jess’ diffusion cell I set up a matrix to test “treated” and “untreated” SC269 samples with diffusion of rhodamine (2mM) and fluoroscein (2mM). I let the experiment sit overnight and analyzed the results this morning.
In the above assay image the samples labeled SC269 are the following from left to right: “untreated” rhodamine filtrate, “treated” rhodamine filtrate, “untreated” fluoroscein filtrate, and “treated” fluoroscein filtrate. As shown above rhodamine and fluoroscein diffused across the SC269 samples in both the “treated” and “untreated” case thus verifying that the pores in SC269 are open.
Based on my analysis the final concentration of the “untreated” rhodamine filtrate was ~0.06mM while the “treated” sample had a final concentration of ~0.08mM. The fluoroscein filtrates had a final concentration of ~0.11mM for the “untreated” case and ~0.13mM for the “treated” case. Note: the value I mantioned above are 2x the values in the plot because the 60uL filtrate samples must be diluted to 120uL in order to measure absorbance in the TECAN.
Today I plan to setup another diffusion matrix testing “treated” and “untreated” SC269 samples with the diffusion of DDM and BSA. My hope is that the results will be similar to those obtained with SC267.