Solid State Nanopores in Biosensing
Multi-Nanopore Force Spectroscopy for DNA Analysis
Control of DNA Capture by Nanofluidic Transistors
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Burst Pressure Tests: Rectangular Windows
Simpore produced a wafer of 50 nm NPN with a matrix of (4) widths X (5) lengths Widths of 100 um, 300 um, 500 um, and 700 um Lengths of 1 mm, 1.5 mm, 2 mm, 2.5 mm and 3 mm Each chip has one membrane. These are the dimensions of the membranes. Using the burst…
w302 Permeability
Wafer 302 appears to have no pinholes and held up pretty well in the centrifuge, up to 5psi for many hours in a wet-wet format. Here are some quick numbers on flow rate over 2 hours, 3.3psi, including radial position:1x PBS (no protein)68 uL (r=4.1)91 uL (r=4.1)99 uL (r=4.5) Using Jim’s new equation. 99uL of…
HUVEC morphology recovers on pnc-Si
Since HUVEC morphology hasn’t looked good in short-term adhesion assays recently, I analyzed HUVEC on the 2nd day after cell seeding. Normally, CMFDA staining is done for 30-45 minutes in serum-free media. I wondered if the serum-free environment during staining was affecting the HUVEC. So, I stained one sample with 5uM CMFDA in serum free…
15nm oxide influence on air permeability
The graph below is air permeabilities for four samples from wafers 344,338,342,345 without and with 15 nm thick deposited oxide on the front side. All of this samples don’t have pinholes.Look at the data for samples 2 and 4 on the graph. The approximate porosities of these samples are 2.7 and 4.6% which is the…
Applying a Voltage to Silver-Coated SNM Can Change the Rate of EO
Because I’ve been having such a difficult time measuring consistent zetapotentials (a big and heartbreakingly inconclusive post on those efforts is forthcoming) I decided to use one of my silver-coated SNMs as an EO pump and see if changing the voltage applied to the membrane changed the rate of EO. And I’m very happy to…
Charged Based Protein Separations
Separations were performed at 2 different salt concentrations with equal volume filtrate and retentate (to avoid dilution problem). Separation time was 40hrs rather than the usual 24. The lower salt concentration run has a lower cutoff – which is just like what we see in the DNA separations. Remember though that proteins have different charges…