During the last weeks of my rotation, Tejas and I attempted to study neutrophil migration in the following setup, using fMLP as a chemoattractant: In order to do this, we permanently bonded a 1 mm PDMS gasket to the bottom and top sides of a 3 micron microporous SEPCON and filled the back side (which is…
I’ve been on a Sepcon assembly binge, and I thought I would post my assembly statistics. On 12/15/13 (assembly “L”) I broke 28 chips out of wafer 1070. 3 chips were visually damaged by the break-out process, 1 was already defective. After inserting the chips into the sepcon housing, testing with 5 psi nitrogen revealed…
Just to give a brief background, I have been working with endothelial cells to make a microvessel mimetic device, and one of the important requirements for endothelial cell culture is to be able to grow the cells under physiological levels of shear stress. Further, since I am trying to achieve neutrophil transmigration through the endothelial…
Etch Rate in BOE 10:1 Thermal Oxide TEOS PECVD Oxide LTO Sputtered 10:1 BOE at 20C 66 nm/min 266.4 nm/min > 180 nm/min * Each oxide type was placed in BOE w/ surfactant for 10 seconds, then the rate per minute was determined. *after 10 seconds no oxide remained on LTO sample therefore the exact…
During the recent visit of Greg Madeijski to our Institute of Biophysics, Imaging & Optical Science (IBIOS) here in Nottingham we had the opportunity to try imaging cultures of cells on several nanoporous membranes, including CytoVu’s and also the unsupported TEM grids without SiN. Here is a short summary of our adventures, along with some…
In the field of gas permeance there is a plot called the “Robeson Plot” (the paper that debuted it) that is used to compare the separation behavior of various membranes made of different materials and measured in different systems. An example is pictured: Various literature data for the log permeability of H2 gas versus the…