Super useful conversions between volumes and lengths
- 1 m^3 = 1000 L
- 1 cm^3 = mL
- 1 mm^3 = uL
- 1 um^3 = fL
- 100 µm x 100 µm x 100 µm = 1 nL
- 100,000 nm^3 (50 nm tall cylinder with 50 nm diameter) = 0.1 aL
This figure appears in our 2016 JMS paper and describes the clogging behavior of NPN with a 37 nm average pore size. The y-axis is the volume of filtrate after 1 min of forward centrifugation at 690g (3000 RPM). The x-axis is particle concentration in #/ml. The particles are polystyrene. Note we chose a size…
Hi all, Today I would like to briefly mention the important parameters to consider when saturating a substrate with proteins by simple adsorption. More specifically, the concentration and time required to fully saturate a substrate ares estimated for systems with different geometry (see the PDF attachment): [NRG] surface adsoprtion The first-order approximations entailed in the above…
Knowledge of the particle number is critical in many of our applications. In trying to rationalize membrane ‘clogging’ by particles for example, it is wise to compare the particle density to pore density at the membrane. When conjugating protein to NPs, it is important to work in a molar excess of protein. In using nanoparticles…
Wright, E., Miller, J.J, Csorda, M.. Gosselin, A.R., Carter, J.A., McGrath, J.L., Latulippe, D.R., Roussie, J.A. (2020) Development of Isoporous Microslit Silicon Nitride Membranes for Sterile Filtration Applications Biotechnology & Bioengineering https://doi.org/10.1002/bit.27240 DesOrmeaux, J. P., Winans, J. D., Wayson, S. E., Gaborski, T. R. , Khire, T. S., Striemer, C. C., and McGrath, J. L….
Introduction As discussed in a previous post, we are working to adapt a PDMS flow insert as a manufacturable component. While our priority was maintaining the original flow channel geometry as Mansouri et al. designed, we slightly changed the channel length (5 mm –> 4.6 mm) to accommodate an extra bonding area for the pressure-sensitive…
Introduction A recent collaborative publication from RIT developed a modular µSiM to address the lack of physiological fluid flow in conventional open-well devices (Figure 1). The magnetic latching assembly of this design allows for quick configuration into a fluidic setup. The flow module is a microfabricated PDMS insert that contains a channel over the membrane…