Super useful conversions between volumes and lengths
- 1 m^3 = 1000 L
- 1 cm^3 = mL
- 1 mm^3 = uL
- 1 um^3 = fL
- 100 µm x 100 µm x 100 µm = 1 nL
- 100,000 nm^3 (50 nm tall cylinder with 50 nm diameter) = 0.1 aL
Wright, E., Miller, J.J, Csorda, M.. Gosselin, A.R., Carter, J.A., McGrath, J.L., Latulippe, D.R., Roussie, J.A. (2020) Development of Isoporous Microslit Silicon Nitride Membranes for Sterile Filtration Applications Biotechnology & Bioengineering https://doi.org/10.1002/bit.27240 DesOrmeaux, J. P., Winans, J. D., Wayson, S. E., Gaborski, T. R. , Khire, T. S., Striemer, C. C., and McGrath, J. L….
This figure appears in our 2016 JMS paper and describes the clogging behavior of NPN with a 37 nm average pore size. The y-axis is the volume of filtrate after 1 min of forward centrifugation at 690g (3000 RPM). The x-axis is particle concentration in #/ml. The particles are polystyrene. Note we chose a size…
Instantaneous Clearance: Kd CBi, and CBo are the concentration of solute in the blood at the input and output of the dialyser. CDi and CDo are the concentration of solute in the dialysate at the input and output. QBi, QBo, QDi, and QDo are the flow rates at the inputs and outputs. The volume of…
When we coat surfaces or particles with proteins we often wonder, ‘how long until the coating is done? ‘ In this 2009 paper we addressed the question directly. We soaked 100 nm polystyrene particles in 10% serum (about 6 mg/ml protein) for different periods of time, washed the particles to remove unbound protein, soluablized the…
Darcy’s Law relates the volumetric flow rate Q through a porous media in response to a pressure gradient . … Where is the intrinsic permeability of the media, is the fluid viscosity, L is the length of the media that fluid must travel through to exit, and A is the cross section normal to the…
Hi all, Today I would like to briefly mention the important parameters to consider when saturating a substrate with proteins by simple adsorption. More specifically, the concentration and time required to fully saturate a substrate ares estimated for systems with different geometry (see the PDF attachment): [NRG] surface adsoprtion The first-order approximations entailed in the above…