Manufacturer’s Data
I repeated a previous experiment but this time I used samples (no RTP) from W685 in oven @ 37C in DMEM(-FBS). Four type of samples (blank chip with no slits, chip with both intact membranes, chip with one intact membrane and chip with both broken membranes) were used at different time points. Again the expected…
Introduction: After meeting with Tucker, he showed me the experiment he conducted to induce membrane transfer to, supposedly, any kind of surface. The method he used was initially with breath, and then he passed on to deionized water vapor. The results were not satisfactory because of the wrinkles on the membrane and not much uniformity…
We know: W683 has no visible pores, W685 has usual porosity, and the recent Wafer 747 is ridden with pinholes. I wanted to test this new wafer for discoloration in comparison to the other two and got the expected result. In the figure below, there are two sets of images of same samples taken at…
The following changes were made in this adsorption study: The MicroCons/NanoSeps were not spun. We will just characterize the differences between the materials. The pnc-Si membranes were assembled into SepCons because of the potential adsorption to the plastic surfaces. Mass standards were run on the gel in order to allow the measurement of protein concentration….
Upper and lower gaskets used to make a counter flow device. The danger is that the enclosing pdms can easily flex into the membranes on the flat side of the chip. A hard plastic cover can be used to prevent accidental contact between the PDMS and membrane.
I ran a few experiments using the stir cell on the shaker table. Diffusion occurs faster on the shaker, but more air bubbles form around the membrane, thus preventing further passage of rhodamine. The first experiment I ran was with .2mM rhodamine diluted from 2mM in a PBS solution. For all of the experiments PBS…