Last week, I looked at discoloration with different wafer numbers (639,670,672,683,685,702,703), pre- and post-rebuild. This experiment was done in the oven at 37C, in PBS and without RTP. Based on the starting color, w670 and w672 are the thinnest membranes. These discolored rather quickly (1 day). The only other membrane that discolored over 3…
I’ve prepared a few slides of discoloration data for tomorrow. The images didn’t copy over from the blog very well, but I think they are good enough for a discussion. labmeeting_oct211
More discoloration experiments. No wafers were RTP’ed, and I looked at different wafer numbers. For each experiment, I looked at discoloration in the oven at 37C and the incubator. The labelling might be a bit confusing. For the top right pictures, I looked at 4.4 mg/mL KHCO3 (left chip) and 0.35 mg/mL NaHCO3 (right chip)….
A couple weeks ago, I posted some pictures that showed a non-linear “dose-response” curve for NaHCO3 and discoloration in the incubator (post). I repeated this experiment at 37C in the benchtop oven. These pictures show that there was a NaHCO3 concentration dependence on the discoloration rate in the oven. In the 2nd set of…
Since Jess saw discoloration last week within a day in PBS with the new material, I did a couple of discoloration studies this week. I looked at wafers 670 and 673 and compared them to 625, 704 and 680-T. These were not RTP’ed, I did the tests at RT since that’s the temp diffusion studies…
I got back on the AFM today to rescan the wafers from yesterday and too scan a wafer that Dave had given me a few weeks ago. The 2 panels are from the height channel, I used the 240 tip (yesterday I used the 160 tip). The top panel is the wafer from Dave and…
I posted some pictures yesterday from AFM last week. I got back onto the AFM today and re-scanned the megasonics cleaned virginia semiconductor wafers. For each image, the top and bottom panels are the height and ZSensor scans, respectively. All scans are 10um x 10um. I did a linear analysis of the ZSensor data for…
I got some wafers from Chris last week(?) that had undergone different Megasonics cleaning treatments by the manufacturer. We’re trying to figure out if these treatments are actually cleaning the wafers. I’ve been having trouble getting the AFM to stay in the “right” mode for imaging with these wafers. However, I have gotten some images….
I did a quick experiment in order to show everyone what the Tecan allows us to do. I made a dilution curve of rhodamine 6G in PBS and measured the absorbance and fluorescence with the fluorimeter. I first did an absorbance scan and found that the maximum absorbance was at 530nm (panel A). Then I…
We’ve been focusing on bicarbonate ions as one of the causes of discoloration. So, I made several sodium bicarbonate solutions and tracked discoloration of non-RTP’ed samples. I made 3.7 mg/mL NaHCO3 (=44mM, this is the concentration in DMEM), 1.85 mg/mL NaHCO3, 0.925 mg/mL NaHCO3, 0.4625 mg/mL NaHCO3 and 0.23125 mg/mL NaHCO3. I also ordered a…