Discoloration presentation

Josh has been kind enough to fabricate a shadow mask for me to create interdigitated electrodes without the hassle of photolithography and etching. In order to be able to use this method, I want to make sure the produced electrodes are the same or better than the old method. I worry about shadowing effects due…

I set up separations with 100kD cutoff Microcon (cellulose) membranes and protein standards.  Here are the results: Due to shorter than normal gel, the myosin didn’t get resolved.  However we can see a separation that appears to be fairly sharp.  We have seen this in the past with cellulose.  The different salt concentrations don’t appear…

One of the hidden secrets of endothelial angiogenesis assays on Matrigel is that non-endothelial cell types also form 2D capillary-like structures (tubes) on those gels.  Motivated by this observation, I wanted to look at fibroblast behavior on pnc-Si transwells, specifically with respect to whether or not they formed vacuoles.  Just to clarify – although Anant…

Summary I need to replace the function of my breath to get water into the femtoliter sized cavity of the monolithic device. I achieve this using a small stream of ethanol vapor as a precursor, and then replace the ethanol with water. Once the chips are wet, they should be reasonably stable until they can…

  Background: In a prior post I showed that we had seeded cells on both nano- and micro-porous SiN Liftoff Membranes. The nanoporous proved to be rather weak and showed numerous broken windows after seeding the cells.   Summary/Conclusions: In this follow up post, I seeded adipose derived stem cells on 50 nm thick microporous SiN liftoff membranes with…

YAP/TAZ YAP/TAZ intracellular localization, and subsequent transcriptional activity, has indicated its role in pathways of mechanotransduction. Investigation of the effects of substrate stiffness on MSC growth and differentiation point to YAP/TAZ as an indicator of how cells are perceiving their mechanical microenvironment and a regulator of the cells’ response to this environment. MSCs cultured on stiff…