Discoloration presentation
I’ve prepared a few slides of discoloration data for tomorrow. The images didn’t copy over from the blog very well, but I think they are good enough for a discussion.
I’ve prepared a few slides of discoloration data for tomorrow. The images didn’t copy over from the blog very well, but I think they are good enough for a discussion.
This is a followup to my previous post, which can be found here. Previously I reported that fluorescein diphosphate (FDP) incubated on the surface of an NPN chip would adhere well enough to be visible on the membrane after gentle washing and treatment with a fluorescence-activating enzyme (alkaline phosphatase, AP.) I also reported that pre-incubation…
Here is an attempt at the active area figure using the “newer” EO membranes (SC172). Here is the raw flow data for the three membrane sizes: If we take the slopes from this figure, we get the flow rates in uL/min. We can then plot against active area: We get a pretty nice fit here,…
As the title states, this post is about shifting an arbitrary pore distribution in an NPN layer. Why would we want to do this? We believe that the underlying pore distribution and shape influences the properties of many of our devices (my SERS sensor work included). Jim has coined the term ‘bowl-with-a-hole’ to describe the…
As seen in prior NRG post, ozone treated samples recover over about 24 hours. However, recent experiments at Gaborski Lab show high variability when using the Corona wand to treat samples with ozone. The goal of this experiment was to determine the contact angles of PDMS samples when exposed to ozone treatment. PDMS samples were…
A quick recap of the goals and initial experiments: https://trace-bmps.org/electrospinning-aligned-polymeric-fibers-on-parylene-stensils/ After obtaining a reproducible optimized fiber deposition settings and an improved device design, we moved on to growing HUVECs on these devices as the next step. As previously stated, given that there is a parallel project with membrane masking of ECM with parylene stencils, we…
I’ve been culturing RPE cells over here in Rochester for my MgF2 fabrication paper, in which the goal is to make a permeable Raman compatible cell substrate. My previous work had shown polarization of RPE cells (via-ZO-1 staining) on nanoporous MgF2 nanomembranes, but in the meantime, I have reworked my MgF2 substrates to be thicker…