Discoloration presentation
I’ve prepared a few slides of discoloration data for tomorrow. The images didn’t copy over from the blog very well, but I think they are good enough for a discussion.
I’ve prepared a few slides of discoloration data for tomorrow. The images didn’t copy over from the blog very well, but I think they are good enough for a discussion.
Hi all, This post showcase a simple (low-tech) alignment tool for assembling the different components of a microfluidic chamber. Essentially, the alignment pieces are made from two index papers cut using the Silhouette Cameo. The two cut index papers are bonded together using double-sided tapes. The square holes are where the membrane chips are to…
This is a short review of a few experiments that Tom had set up this morning. Carbonized membranes: A few carbonized membranes that were used for permeability measurements were tested in EO. The membranes were left in the sepcon, which was filled and placed in a reservoir. Electrodes went inside the sepcon and the reservoir. …
I am working with different electrode materials as a substitute for ITO for my TEER measurement device. I used silver-silver chloride electrode and platinum-iridium ribbon (90:10) for that purpose. I made AgCl electrode by dipping about an inch of silver wire in silver chloride ink and letting it dry for half hour. I obtained the…
Type I collagen is a fibrous protein and the largest constituent of the ECM by mass. Collagen fibers are aligned across several millimeters to centimeters within load-bearing tissues, like the musculoskeletal tissue, cardiac tissue, and the cornea. Millimeter scale regions of fiber alignment are also observed in the tumor stroma and fibrotictissue. At the cell scale,…
While I have been making nanoporous MgF2 for a while, it has become obvious that the strength of the material is handicapping our ability to generate data; as the membranes rupture we lose weeks of cell culture work. While we have generated some interesting Raman and cellular effects using the nanoporous MgF2 membranes, they are…
On Day 2 of cell growth from trial 3 of adhesion study, cells looked much more spread out but less in density. Most of the cells were observed to have concentrated near the edge of the pnc-Si chip near the Sepcon as seen in the figure below (first image in the bottom row). This could…