Last week, I looked at discoloration with different wafer numbers (639,670,672,683,685,702,703), pre- and post-rebuild. This experiment was done in the oven at 37C, in PBS and without RTP.
Based on the starting color, w670 and w672 are the thinnest membranes. These discolored rather quickly (1 day). The only other membrane that discolored over 3 days in PBS was w702. So, wafers from the rebuilt system may not be inherently more unstable than older wafers.
Hi All, This post is more of a dump of figures that I have put together for Dan’s and my upcoming Journal of Membrane Science manuscript. I have included figures and captions, so any feedback would be greatly appreciated.
Previously, we showed that our method of pll-peg coating is effective for creating non-fouling substrate which can prevent protein adsorbtion, and this method can be used to create a non-fouling pattern on the surface. In the next step, we wanted to make sure that whether our peg coating is effective for preventing cell adhesion or…
Production this week yielded mixed results. We ran eight wafers through four different RTP temperatures with/without the susceptor. We wanted to test our theory that temperature non-uniformity inside the chamber was causing the morphology change across the wafer. This proved to not be the case! Wafers that were treated with and without susceptor showed the…
We have often encountered cytochome C dimers as part of our work. For example in this figure from Jess that we often refer to in our hemodialysis work. It turns out that Cyto C polymerization is a well known phenomenon. The dimers are very tough and survive the denaturating conditions in Jess’ gel. Here is…
I got back on the AFM today to rescan the wafers from yesterday and too scan a wafer that Dave had given me a few weeks ago. The 2 panels are from the height channel, I used the 240 tip (yesterday I used the 160 tip). The top panel is the wafer from Dave and…
As discussed in a previous post “Clearance, Kt/V, and Concentration…”, we will use the Kt/V metric to measure the effectiveness of dialysis. There are other methods that are used: one of these is the Urea Reduction Ratio, URR. URR is a fractional decrease in Blood Urea Nitrogen, BUN, in a single dialysis session. It is…
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Which #’s are pre and which are post? Also this is done at 37C. Jess’ discoloration was at RT. We expect the problem to be worse at 37C, right?
I actually can’t keep track of which are pre and post build. I think all of them except w639 are post-build.
Should be worse at 37C compared to 25C.
Barrett’s correct, all wafers except 639 are post-rebuild.