UPDATED 12/12/07 – Added data on albumin. In these gels, the band from beta-galactosidase appears to overlap with phosphorylase b making it difficult to determine which protein passes and which is retained. I have used Mike Bindschadler’s pore imaging software to find the pore sizes of the three membranes I have use to perform succesful…
Today I looked at 3 more wafers: w158 (9/7/07) – some pinholes, overwhelming amount of very fine debris, not cbs as far as I can tellw160 (9/7/07) – some pinholes, debris, no cbsw171 (9/26/07) – many pinholes, lots of debris, no cbs w158 (200x) – small particles Link to updated report.
Two simple diffusion separations were performed before break using membranes from wafers 205 and 213 (locations unknown as these were salvaged/misplaced chips). I ran the samples I collected on a 10% SDS-PAGE gel today. It seems that the membranes were able to separate the standards at two different cutoffs. The missing band in the middle…
I stripped and viewed samples from wafers 186, 185, 184 and 182. None of these samples had cigarette burns. Follow this link to the updated summary: cb_report Previously I had noticed cigarette burns (small ragged pinholes) on samples from wafers between numbers 202 and 216. All of these wafers had been stripped using a full…
This is a 12% SDS-PAGE gel of the human albumin (alb) and cytochrome c (cyt c) EQ tests. Both alb and cyt c passed through the membrane, while the cyt c impurity (middle band) and alb impurities (high bands) did not. The next step? I’d like to try an albumin/IgG separation with membranes of similar…