During the last weeks of my rotation, Tejas and I attempted to study neutrophil migration in the following setup, using fMLP as a chemoattractant: In order to do this, we permanently bonded a 1 mm PDMS gasket to the bottom and top sides of a 3 micron microporous SEPCON and filled the back side (which is…
This is just an update for the experiments Tejas and I have been working on for the past two weeks: First, we tried incorporating 20 μl of Geltrex (10 mg/ml, diluted with DMEM from stock concentration: i.e. 15.6 mg/ml) in the back of a non-porous chip with 0.02 μm blue fluorescent nano-spheres (1:10 dilution from stock concentration)….
For the past month, I have been working along with Tejas towards incorporating an ECM-like hydrogel into the back (edged side) of a pnc-Si chip. The purpose of this effort is to add another layer of complexity to Tejas’ in vitro microvasculature system, in order to study neutrophil migration across the endothelium. We first pondered…