The goal of the blood brain barrier device is to co-culture cells in a closed system, with the apical chamber under flow conditions, while still being able to access either chamber for media changes or to introduce new solutions. Electrodes to measure TEER will need to be included in future design iterations. There are two…
One aspect of the blood brain barrier device is that it be a closed flow system. This would not only better mimic an in vivo environment, but also maintain Sema4D activity, negatively affecting endothelial cell junctions and providing a target for the antibody. The microporous membranes were not yet available, so a closed flow system…
A quick recap: Vaccinex has asked the lab to design a closed system device with the capability to co-culture on microporous membranes. This device would be used to mimic the blood brain barrier, with endothelial cells cultured on the apical side and glial cells cultured on the basal side, and would allow for the testing…
Vaccinex has developed an antibody, VX15/2503, for Semaphorin 4D, a molecule implicated in axonal guidance mediation and blood brain barrier (BBB) disruption. The role of SiMPore in its partnership with Vaccinex is to produce a workable model of the BBB, with the ability to introduce flow conditions and measure an electric gradient across the membrane. …