Discussion: The BSA may have not been fresh enough to give good data, the difference between background and signal are pretty low. The Rhodamine IgG gave better signals but the PEG4 treated SiN is between the untreated SiN and bare pnc-Si. The latter having a lower signal. Here are the details. The fluorescent BSA was…
What I had in my two previous posts described as permeability graphs were in fact total volume passed/ total time passed graphs: What I thought I saw was that cake layer formation was dependent on the filter geometry. There is some justification for such a dependence in the literature – These folks found that spacer…
At some point, when things settle down, I’ll create a page dedicated to the TECAN machine and document all of the troubleshooting and tricks I’ve learned so far. Before that happens I have another mystery to solve. Take a look at the following three scans: This first are some scans of BSA filtrates from…
These volume measures were conducted at 5.6 +/-0.2 PSI using the variable area sepcons with either a half slot, one slot, three slots, or the normal five slots. Note that the pressure was normalized via a pressure regulator, and this normalization was confirmed with a manometer. Filtrates were collected in a series of the…
Katia in Dr. Shestopalov’s Lab functionalized some pnc-Si windowless chips. The process she follows is shown in Figure 1. She provided chips at various stages of the process. “Grignard”, “Diazirine”, and “PEG”. After functionalization, the chips were wetted with 20 µL of 1:1 pbs then 40 µL of 5 mg/mL f-BSA was added. The…
Regarding the single channel dialyzer chip with 20-nm thick nano-porous silicon membrane: 10 mm long, 300 µm deep, 500 µm wide channels. Now that we’ve shown that a measurable quantity of urea is filtered out of DI water in one pass through the single channel dialyzer chip, we turn our attention to “Things Which Must…