This is my first attempt to grow astrocytes (NG108-15 cell line) on PET and pnc-Si transwells.  These are passage 8, seeded at 50000 cells/cm2 and grown for 7 days.  Of the 8 pnc-Si transwells (RTP’ed SC104) I made, all but one survived this 7-day cell culture experiment.  These cells were plated on the bottom on…

In my previous post, I showed images of bEnd (P8) cells on PET and pnc-Si transwells.  These were seeded at 105 cells/cm2 and grown for 14 days.  This post contains the TEER data.  I simply normalized all values to the Day 0 (no cells) resistance, so everything starts at 100%. This data for PET matches…

I continued to run some of the samples that I described in this post last week.  These are P8 bEnd3 cells, seeded at 100000 cells/cm2 (just below confluence) and grown for 14 DAYS.  The pnc-Si was SC126 that was RTP’ed.  I measured the TEER of these samples for 2 weeks and then stained the cells…

Using the same procedure as in the last post, I was able to simulate a 6um thick membrane with the same pore characteristics as the experimental pnc-Si membranes.  Material diffuses more slowly through this membrane, but not as slowly as I expected.  The following figure shoes the filtrate/retentate concentration ratios at 24 hours for a…

Last week, we ran several wafers with a silicon target from Refining Systems (Las Vegas).  In addition to the source material being different, the target bond was a silver elastomer vs. the typical indium bond that AJA uses.  Below is a TEM micrograph of a comparison between the RS silicon and AJA silicon.  Both these…

Last week, Barrett and I made up the three buffers needed to perform this test: Dissociating buffer: 0.1 M glycine-HCl, pH 3.5 1 M Tris Binding buffer: 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.5 In addition, I made a solution of protein A beads that consists of 0.0166 g of beads in 50…

Follow up to this post.  As a brief reminder, I have performed 3-D simulations of separations in large and small volume systems.  I have defined an effective diffusion coefficient that takes into account the membrane hindrance and compared this to free diffusion.  As the Deff/D0 ratio drops, this means the membrane has a higher impact…

With the previous 2 experiments, I made a couple of 6-slit pnc-Si transwells.  These chips were from the cell culture wafers I had made a while ago.  These samples (SC126) were RTP’ed before use. My improved technique, combined with the RTP, enabled me to keep cells growing on these pnc-Si samples for 1 week WITHOUT…

Along with the PET data I just posted, I also examined cells on pnc-Si transwells.  The following images were taken at 20X (through the bottom of a 24-well plate), and the cells are passage 8 bEnd3.  They were seeded on the membrane side @ 100000 cells/cm2, and then the transwells were flipped for growth.  Images…

A while ago, I posted some images of cells grown on commercial polyester (PET) transwells.  Here’s the post.  I found that there was an annulus of dead cells around the edge of the transwell when cells were grown on the bottom of the membrane.  I contacted Corning and they said that these dead cells were…