These are cross sections of my comsol diffusion model. Each chip has 6 square wells terminated in a membrane. This cross section cuts across the two middle wells. Concentration contours are in 0.025 intervals.
6 hours:
12 hours:
18 hours:
24 hours:
One of the major goals of my work with capturing exosomes is to show that our purified population is less contaminated with protein than exosomes purified by ultracentrifugation. This is important because the protein (and all the other junk floating in plasma such as cfDNA) can cause problems with identification and characterization of the exosomes….
Harold Smith at the Med Center proposed an experiment for Jess and I to try, in which we use our membranes to separate proteins onto a PES, PVDF (Polyvinylidene fluoride) or Nitrocellulose membrane, similar to a Western Blot of DNA microarray. The idea is to create a system to screen for drugs that break protein-protein…
Updates: We can now put the glass on top of the mask, which allows the use of nonstick agents in the creation of PEG microarrays. The ability to cross-link PEG-Diacrylate (PEG-DA) with a photoinitator and UV exposure was previously tested and shown to work. At the lowest power setting on the Leica (~25%) fluorescence source, we were…
I was concerned that even though my calculations for etching the microporous MgF2 material show that the silicon nitride was being removed completely, not all of the silicon nitride was indeed being removed. Hypothetically, the presence of additional remnants of silicon nitride could be causing additional film stress and cause membranes to break more frequently. I…
I’ve been thinking of demos/experiments to do with my upcoming class and one of them is SEM. I was thinking of handing out some unknown nanoparticle solutions and have them figure out the size and maybe material by SEM, AFM and/or DLS. I’m approved to run the SEM now, so I hopped on today to…
Word Document: Blog Write Up 4.8.19 Over the past two weeks I have been working on creating a protocol to eliminate the organic debris from environmental water samples, leaving only microplastics behind on the silicon filters. The goal for this is to be able to really isolate out just the plastic in environmental samples making…