These are cross sections of my comsol diffusion model. Each chip has 6 square wells terminated in a membrane. This cross section cuts across the two middle wells. Concentration contours are in 0.025 intervals.
6 hours:
12 hours:
18 hours:
24 hours:
Dave Fang posted a poster in 2010 about particles found in DI water. I encountered the same issue during the nano-pocket permeability experiment. He said, “I’ve become increasingly suspicious of membrane clogging during the … permeability experiments.” I conducted an experiment with ultrapure water (UPW), typically filling a 1L glass container from a UPW machine….
W705 performed well in terms of permeability and separation of Hybrid Silica Nanoparticles. No water passed in the wet/dry format (no matter ozone or testing configuration – centrifugal or pressure cell). Reasonably high flow rates when tested in wet/wet configuration in the centrifuge. PVP enabled flow in the pressure cell in an otherwise wet/dry configuration….
Tejas found this… A cool article and video on water ‘sticking’ the surface. Notice the difference in advancing and receding angles. http://web.mit.edu/newsoffice/2013/droplet-surface-adhesion-0219.html
The following post is the abstract that I recently submitted for the BMES Annual Conference. Introduction: Permeable membranes provide a physiologically relevant cell culture substrate for in vitro study of barrier cell types, such as endothelial cells, which assume a polarized morphology with distinct apical and basolateral surfaces. Cell morphology and subsequent monolayer formation and…
One of the other suggestions from Angela Glading at lab meeting a while ago was to quantify the vacuoles on different culture areas. So, I selected images from 3 different experiments (all at day14 of growth) and counted the number of cells which expressed vacuoles on different areas. I then used my mask code in…
I set up separations of 5nm Au nanoparticles in both H2O and KCl in a new system. The stir cell isn’t always reliable for separations due to leaking and contaminating (?) particles from the plastic. To perform these separations, I added 20ul of sample to a sepcon and wetted the backside of the sepcon with…