We will devote the first NRG meeting of each month to journal club. First up is Karl who will review two papers describing the use of single nanopore membranes as a method of characterizing particle size and zeta potential on a particle by particle basis. The technology produces intriguing data sets like the one shown in the right panel here:
The two papers Karl will discus:
Also check out the company commercializing this technology: iZon
Purpose: We want to know the effects of the RTP process on pore size, pore density for the biased films coming out of the AJA. I believe that the RTP ramp rate is important for forming bigger pores. Some work (Joe Qi Thesis) has indicated that the pores come out more circular as the wafer is…
YAP/TAZ YAP/TAZ intracellular localization, and subsequent transcriptional activity, has indicated its role in pathways of mechanotransduction. Investigation of the effects of substrate stiffness on MSC growth and differentiation point to YAP/TAZ as an indicator of how cells are perceiving their mechanical microenvironment and a regulator of the cells’ response to this environment. MSCs cultured on stiff…
Josh has just started teaching us how to play with these; some early findings: – So far, standard centrifugation results mostly on the caking of lipids that clogs the membranes. – Use of the pressure cell has shown some promise, we have passed 120nm lipid-nps through 400nm pores @10PSI. The result? 70nm particles… – Reverse…
Dave and I characterized the membrane samples using the 4-point probe system at Kodak research Lab. It is NOT the previously discussed micro-4-point probe. The spacing between two probes is about 2mm. We first tested a quarter bare wafer#388 at five points (see the diagram below). The average sheet resistance is around 80 ohm/square.sites (1)…
This week, I did a quick discoloration experiment based on Chris’s comment at lab meeting. He wondered if soaking the chips in water for a while would allow potential discoloration-causing contaminants to slowly leech out. I took 2 chips from wafer 622 and soaked them in ~ 300mL DIH20 overnight (with a couple of water…
My Talk (PPT; Keynote) Program My Notes
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Here is a review that discusses the various applications for nanopores. It cites the Striemer et al. 2007 Nature paper (ref #16) as an example of ultra-filtration using nanopores.
http://jla.sagepub.com/content/15/3/243.short
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