We will devote the first NRG meeting of each month to journal club. First up is Karl who will review two papers describing the use of single nanopore membranes as a method of characterizing particle size and zeta potential on a particle by particle basis. The technology produces intriguing data sets like the one shown in the right panel here:
The two papers Karl will discus:
Also check out the company commercializing this technology: iZon
Objective: Determine the minimum required area and number of exploratory cytoplasmic protrusions for sensing ECM alignment and causing cell polarization. Hypothesis: Cells only need to sense collagen alignment through exploratory protrusions extended during initial phases of cell seeding and not throughout the entirety of the cell, and a minimum number or size of protrusions is…
Just in case you missed this one: www.nature.com.ezp.lib.rochester.edu/nnano/journal/v2/n11/full/nnano.2007.354.html This article makes the the previous papers seem mundane. It has inspired me though – maybe we can create a computer simulation that will demonstrate that pnc-Si membranes will dance and sing songs under the correct conditions. I will leave the choice of genre to the programmer…
Hopefully in the next week we will be submitting the following manuscript … X. Li, D. Johnson, W. Ma, H. Chung, J. Getpreecharsawas, J.L. McGrath, A.A. Shestopalov, Modification of Nanoporous Silicon Nitride with Stable and Functional Organic Monolayers, ACS Chemistry of Materials (2016) submitted. Here are the highlights … Traditional silanization of silicon materials uses…
As we were conducting our experiments on dimpled Sylgard substrates we knew that another micro-topography that we wanted to investigate was micro-pillars. In like manner to the dimples, a post microtopography should provide some sort of surface for the cells to “grab onto” and spread as they would on any ECM in vivo. These…
The purpose of this device is to make uniform and straight scratch wounds in a 96 and 24 well plate for migration experiments. Previously, scratch wounds were made by hand using a p-10 pipette tip by scraping the tip in a straight line along the bottom of the well. Not only is manually scratching the…
What is SiR-DNA? It is a far-red DNA stain, known as SiR-Hoechst, and can be used for live-cell imaging. Most current DNA dyes are toxic or affect the cell’s motion or proliferation. https://www.nature.com/articles/ncomms9497 It does not affect the migration of human dermal fibroblast cells (hdFb). The motility parameters (speed and displacement) for a 24 hour…
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Here is a review that discusses the various applications for nanopores. It cites the Striemer et al. 2007 Nature paper (ref #16) as an example of ultra-filtration using nanopores.
http://jla.sagepub.com/content/15/3/243.short
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