Solid State Nanopores in Biosensing

Endotoxins (~10-1000kDa) are structural components of bacteria (LPS is the best known) that are released from bacteria when they die.  Endotoxins cause inflammatory responses in humans and animals (infections give you a fever) and in cultured cells.  For most cell culture applications, you don’t have to worry too much about endotoxins.  For monolayer permeability experiments,…

Following up on our previous work with SiN-NP with varied RIE times, Sarah and I measured permeability and permeance using a new measuring system better suited to the higher permeance and permeability we expect with the larger pores and higher porosity. Using the pressure regulator pictured above, we can set pressures of 3 psi and above…

We are working on finishing up a manuscript on our nanosphere lithography work. this post shows some of the figures for said publication. This paper includes work done with 300 and 200 nm NS as preliminary work, and then focuses on 200 nm NS only to evaluate the porous pattern obtained. From the data presented,…

In my last post, I introduced the concept of pore size dependent carbon growth on alumina coated membranes. Here, I have plotted a Gaussian fit of the tail of the pore size distribution of carbonized membranes (with and without an alumina coating). I am only showing the tail of the distribution to highlight the sharpness…

I attempted to clear the large format lift-off devices (from Josh). I was unable to clear, in a reasonable amount of time (< 2 hours) by pumping IPA. So I switched to PBS then Blood and blue-dyed PBS (dye is estimated to be between 10 nm and 500 nm, obviously the amount of dye near…