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(UPDATED with n=4) I’ve repeated the burst pressure experiments performed by Sabrina, with the 75 nm thick hemodialysis chips. The previous chips are 50 nm with a burst pressure of 1.5 psi (10.5 kPa). I found the burst pressure of the 75 nm chips to be 3.62 psi (25 kPa) n=4. Increasing the thickness 1.5 x lead…

I am having some problems with my electroosmosis device and I was hoping that people with more knowledge about the TEM chips might have some suggestions.  For these trials, I used membranes from a wafer labeled 412-S.  I am still using the PDMS device from my last post although now I have mini “clamps” that…

As part of my thesis (and a tie-in to the microplastics research our lab has been conducting) I’ve been working on creating a measure of membrane capacity. A robust way to do this involves using microporous chips and polystyrene beads. Previous experiments on 8 micron slit membranes have shown that phosphate buffered saline (PBS) and…

The physical properties of the extracellular matrix such as stiffness, viscoelasticity, fiber diameter, and chemical composition are well known as important modulators of cell behavior in vivo. Therefore, over the past decade, there has been a major emphasis on characterizing the properties of the in vivo ECM and also engineered matrices in vitro. In this context, properties such…

An application of nanothin and highly porous (NTHP) membranes for mesenchymal stem cell (MSC) differentiation in a co-culture setting. The highlight of this article is that the NTHP membrane allows direct contact of the MSC to cardial myoblast (H9C2) while still maintaining the separation of the different cell types. This ultimately leads to higher degree…