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O-ring position doesn't matter
Christine and I have been looking into whether the O-ring position affects transport through pnc-Si transwells. The hypothesis was that the unstirred water layer under the Sepcon might be diminished by switching the O-ring from its normal position (“O-ring bottom”) at the bottom of the Sepcon to above pnc-Si (“O-ring top” or “pnc-Si bottom”). This…
Neurofilament H Immunofluorescence
For my BBB project, I’ve wanted to get nice images of glial cells and endothelial cells on opposite sides of pnc-Si membranes to demonstrate 1) optical transparency 2) that both cell types are in focus (and thus right on top of each other). I’ve gotten some images like that in phase contrast but I also…
Review of nano-fabrication
Hello everyone, for my first ever post I would like to share with you a collection of ideas that has turned into a review I’ll be working on. Here you will find some selected publications that report interesting fabrication processes at the nanoscale. I have summarized the processes and added some raw schematics of how…
Flow Cell/µSiM Testing – Push versus Pull Flow Modes
Introduction Static endothelial cell cultures have been routinely performed and optimized on the µSiM platform for multiple membrane and cell types. In order to increase the physiological relevance of our device with tissue vasculature mimetics, we need to incorporate fluid flow to emulate shear stresses experienced by endothelium under physiological circulatory conditions. Recently Mehran has…
Success with PDMS-Glass Bonding
ByMike HYesterday afternoon I made a new batch of 10:1 (base : curing agent) PDMS and put it in the oven at 80C for two hours to allow it to cure faster (compared to the three days it takes at room temperature). Once the PDMS was cured Henry used the UV Ozone system to again try…
Cell-substrate interaction modeling
The idea of this project started when we were writing a mini-review about cell-substrate interaction. Although most of studies of cellular responses to porous membranes have pointed towards a multiphasic response with the same trend of changes with respect to the pore sizes, we also found some variation in the results. We suggested that some…