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In order to further test the model, I’ve set up additional rhodamine diffusion assays.  In each case, the setup is similar to the nanoparticle/protein/dna separations –  20 uL of .5 mM rhodamine on the top of a SepCon and 20 uL .1 M KCl on the bottom.  Two different membrane geometries were used so far…

This was something I set up a few days ago and finally got a chance to run the gel.  I was curious to try this after the previous forced separations using brain extract.  If you recall, the “separation” using the pressure cell did not have a visible cutoff and all of the bands appeared in…

Here are the results of a protein ladder separation with w677.  If you recall, 677 is the 200um wafer that did not fit into the SepCons but had very few pinholes. S = Standards Ladder, C = Protein solution that never came in contact with membrane, R = Retentate, F = Filtrate Myosin (Band at…

Here is the figure we talked about in the last meeting. For each specific membrane, I’ve plotted both the experimental permeability and the permeability calculated from the theory. You can see in some cases they are close, but in others they can be pretty far off. Omitted from this chart are retests. Updated: Graph is…

Hi all, This is a “take two” of the mock figure for our manuscript titled “Porous substrates influence early endothelial migration and the associated fibronectin fibrillogenesis”. Many new figures are produced based on the reviewer suggested experiments. It is likely that we will have the word”early” from our manuscript title. PS: The old, raw mock…

If you know me at all, you know I enjoy nothing more than ultrafiltration and Parylene-C deposition. Now these activities are combined into one device to improve the biocompatibility (platelet activation and adhesion) of nanoporous membranes. In this letter, polyethersulfone (PES) membranes were coated with Parylene-C, poly(chloro-para-xylylene) aka (PCPX), while glycerin vapors rising through the…