Overview: The goal of this experiment was to determine which membrane type adipose derived stem cells (ADSCs) would proliferate best on. The ADSCs grew over a four day period and were imaged on days 1, 2, 3, and 4. The cells were counted on each of these days to determine growth rates on each of the…
Below is an optical image that shows the amount of area loss caused by the preferential EDP etch. Based on these numbers, our most recent membrane slits measure 115 um x 2014 um.
The current method of testing water permeability through SepCons is to place a volume of water inside a SepCon and spin it inside a centrifuge. After a set period of time, the volume passed is collected. The “approximate” permeability can be calculated as: volume passed/(pressure*area*time of experiment) in units of uL/PSI/cm^2/min. The reason this is…
I worked with Paul this afternoon and finally we highly suspect the raw material used for the prototype diffusion chamber could be Polycarbonate. Paul could not provide direct evidence for the material source since he did not keep any purchase orders or materials data sheet… However, we did check our chamber with another plastic board…
After our meeting with Professor Dumont on Tuesday it was decided that we needed to try and perform the BSA/DDM seperation with a more porous membrane (to allow for eventual pressurization). SC256 was choosen and as an initial step I ran a simple small dye diffusion experiment to verify that the pores were open. As…
Chris and I got to play on the new Zeiss scanning transmission electron microscope (STEM) today (learn about STEM here). We didn’t get to spend a lot of time on it, but here are some quick images we snapped. wafer 416 @ 25kx (left) and 65kx (right) Even though we’re imaging in transmission mode, the…