Endothelial Vacuoles Induced by Flow

Summary I need to replace the function of my breath to get water into the femtoliter sized cavity of the monolithic device. I achieve this using a small stream of ethanol vapor as a precursor, and then replace the ethanol with water. Once the chips are wet, they should be reasonably stable until they can…

Michael and I have been working to create cell channels of varying widths and heights to test out a concept used in a paper we recently read as a group. This work claims to see cell cords in 12 hours, so no media renewal is necessary.   The channels were made by layering and bonding…

In the past, I have been working on developing mechanisms to inhibit neutrophil migration through endothelial basement membrane. Please find the previous blogs on the following links https://trace-bmps.org/updates-on-neutrophil-collagen-migration-experiments/ https://trace-bmps.org/manipulating-neutrophil-migration-through-the-vascular-barriers/ https://trace-bmps.org/sem-imaging-of-neutrophil-transmigration-through-collagen-gel/   This is a small update on the following. CD29 is beta-1 integrins, which are used by neutrophils and endothelial cells to adhere to basement…

I attended the 2014 Gordon Research Conference (GRC) on Fuel Cells in August. For those of you not familiar with Gordon conferences, I will make a plug for them as a great venue for learning much detail and interacting closely with many of the major contributors in a specific field. GRC has a wide variety…

I decided that instead of trying to figure out why it was delaminating, I would simply remove the option altogether.To that end, I painted PDMS on two NPN chips up to the oxide spacer.   Attempt 1: This chip was one I laminated myself using my breath to contact the membranes. In case it’s unclear,…