Counterflow Dialysis
Hey everyone, thanks for helping me out with my presentation this morning. I posted A LINK to the ppt. below. Let me know if you have any questions or comments. Thanks!
Hey everyone, thanks for helping me out with my presentation this morning. I posted A LINK to the ppt. below. Let me know if you have any questions or comments. Thanks!
In my last post on this subject, I showed that cutting off permeability of pnc-Si chips inhibits vacuole formation in endothelial cells. To do this, I seeded cells on the membrane side, allowed them to attach, inverted them onto a media bubble contained within a cloning ring, wet the well-side with media and then placed…
In my last post, I presented the results of my BBB TEER experiments. For the BBB co-culture, there was a spike in TEER. I hypothesized that this was caused by media acidification by rapidly proliferating glial cells, which in turn negatively affected barrier function of endothelial cells. When I presented this data to Angela Glading,…
BMES 2019 saw a number of talks being presented on the topic of controlling extracellular matrix architecture and understanding how cells respond to physical cues from their in ECM. My focus was primarily on talks pertaining to the tumor microenvironment or other tissues which show anisotropy in ECM fiber alignment such as cardiac tissue. Recently,…
As a rotation student from the Biophysics depart, I’ll be spending the next six weeks or so helping Dr. Stephan Barcikowski use the nanomembranes to do a number of separations as part of a nanofabrication procedure. Dr. Barcikowski makes extremely pure gold and platinum nanoparticles (~10nm) colloids using laser ablation and then bioconjugates them with…
First Try is detailed in this post. Three membranes from w334 were used to separate depleted serum. As before we see that all of the proteins pass through the membrane, and again we can observe the one middle band that seems to appear more in the filtrate (red arrow). It’s possible that most of these…
Simpore produced a wafer of 50 nm NPN with a matrix of (4) widths X (5) lengths Widths of 100 um, 300 um, 500 um, and 700 um Lengths of 1 mm, 1.5 mm, 2 mm, 2.5 mm and 3 mm Each chip has one membrane. These are the dimensions of the membranes. Using the burst…
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Pretty cool Derek.
Anyway to make it full size?
Yup, just click “start presentation” in the upper right hand corner of the page after opening the link.