Counterflow Dialysis

Last week I made a post about the troubles that a toluene-based PEGylation process presents to the PDMS in the CytoVu assembly. It was suggested that this issue might be overcome by replacing toluene with ethanol in the process, but I can report that unless I did something wrong (which is, I suppose, quite possible)…

In the soon to be submitted paper on diffusion models, we list two contributions to membrane resistance.  The first is due to the steric and frictional hindrance a molecule encounters as it enters and diffuses through a pore (Renkin, Deen).  The second is due to the time that it takes for the molecule to diffuse…

Last week during the deposition step, Chris and I discovered that part of the Cu “power cup” (piece that is bonded to target to make thermal contact with sputter gun) had eroded and coated the inside of the target shield with copper. Later EDX and TEM characterization showed that there was indeed contamination in the…

Introduction In severe sepsis, a primary infection gives way to a maladaptive immune response leading to wide spread edema and multiple organ dysfunction. The initial infection can occur in many peripheral tissues throughout the body. The pathogen associated molecular patterns (e.g. LPS on gram-negative bacteria) interact with pattern recognizing receptors on resident macrophages and endothelial…

Do not just send her an e-mail. The form found is found here 

PDMS/membrane deflection was measured on Veeco profiler by JP and me. The main problem with this kind of measurement is that it is taken form the backside of the membrane and the walls need to be excluded from the measurement. Using 20x objective helps but still has some problems. PDMS was first spin-coated on the…