New wafers permeabilty and burst pressures data

Along with differently sized square membranes, I also designed a wafer with different sizes of slits.  On each chip, there is a 4000, 2000, 1000 and 500um long window.  The thicknesses of these windows are 200, 100, 50 and 25um.  I’m interested to see if there is an aspect ratio that causes endothelial cell alignment/tubulogenesis. …

It is of interest to investigate the properties of CO2 transfer into a cell culture media using nanomembrane technologies. Maintaining the pH of the cell culture medium is critical to cell viability. Most cell lines grow well at pH 7.4 and are inhibited by growth at pH 6.8. Glucose is usually the sugar included in…

I guess there was a mistake in the last permeability plot.  The real data for the 4 bar plot should look like this: Unfortunately that doesn’t look so good anymore.  The third bar though is the one that I originally processed to have many more pores (w705).  The reprocessing gets it down in the 8%…

To prepare the BBB devices for cell culture, they must be sterilized. To that end, I wanted to see if there were any adverse effects from autoclaving the device. Recipe 4 on the U of R autoclave is a dry process that we use to sterilize tips, so I used the same recipe. I placed…

I’ve tried to get some more data for the phenomenum that may be electro-osmosis in our electrophoretic cell.  In the last post I looked at the rate of passage of 1x PBS in pinhole membranes from w621.  After that I first tried to see if DI H20 would also work (also pin.  At 15V (which…