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Goniometer Introduction
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RTP Time Variation for Cell Culture
In an attempt to improve membrane life in cell culture, different RTP times were tried on a single wafer. Samples from W514 were RTPed in Ar at 800C for 1, 5, and 10 minutes, respectively. Cells were then seeded at confluence on membranes, with membrane stability monitored for breakage. Discoloration was monitored on samples without…
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Live/Dead Assay for BBB Device
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10 dynes/cm^2 bottom channel Aline
AlineFlowBottomChannelSup AlineFlowBottomChannel
Tecan and Transport
Before starting my transport project with the use of the Tecan, it was important to see how the Tecan worked with the sepcon and transwell kept in the well plate. The goals from this experiment were to: 1. Test for fluorescence from the plastic. 2. See if fluorescence from the apical side has an affect…
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This is all great data. Have we made any progress in mapping the flow rate to the pore size distribution and porosity of the membranes?