This is preliminary data for air permeability in track-etched membranes for membranes with different pore radius.
For comparison here is a table of the performance characteristics for TE membranes
Measured data is of the same order as official TE membranes data.
We have successfully plated adipose derived stem cells (ADSC) on microporous silicon nitride (SiN) membranes. Cells plated on nanoporous SiN appeared fine at time of seeding, but membranes were broken at 2 hours – possibly due to media washing or cell spreading/contracting. Josh Miller made new Microporous and Nanoporous Nitride liftoff membranes. These membranes were…
Production this week yielded mixed results. We ran eight wafers through four different RTP temperatures with/without the susceptor. We wanted to test our theory that temperature non-uniformity inside the chamber was causing the morphology change across the wafer. This proved to not be the case! Wafers that were treated with and without susceptor showed the…
Last week I conducted a variety of experiments to try to make a nanofluidic transistor (a device described in detail in this previous journal club post). I did some light optimization of the E-beam deposition of Ag films over a 3nm Ti adhesion layer in the PVD lesker machine and found that 12nm of Ag gives…
After experimenting with some different coatings and membrane types in single-well CytoVu devices, here are some representative images of the results: SiN (Nitride) membrane with a Geltrex non-gel coating @ 44hr: 10x: 20x: 40x: SiO2 (Oxide) membrane with a Geltrex non-gel coating @ 42hr: 10x: 20x: 40x: Also, some Live/Dead Assay images were taken on…
[Background read – https://trace-bmps.org/blog/theory/2015/01/28/why-teer-values-are-multiplied-by-membrane-area-dc-version] I am putting the excerpt of this old post from Jim in this post. Tom helped me in gathering this data. In order to prove my point that electrical resistance is very sensitive to the membrane geometry, I decided to perform some proof-of-concept experiments with regular transwells. I used 3…
This is an update of my progress with the diffusion model. Sieving coefficients for standard setup (Renkin only) In this image there’s a strong sigmoidal curve for the 24 hour diffusion. However, the cutoff (~34nm) is higher than cutoffs found in experiments. The lower time curves have unexpected shapes. This may be due to…
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At what pressure was the data in the table acquired?