I reran a set of hydraulic permeability tests at 16.56psi (3500RPM) hoping that this would clarify the previously collected data at the 21.63psi (4000RPM) and the 33.79psi (5000RPM) range that did not seem to agree with the trend of the other data points. As shown above this point still does not seem to be in line with the remaining trend of the other points. To compare this to the data from last week…
I am currently at a loss to explain these results.
HUVEC adhesion was tested on different substrates. This time solid chips w/o membrane slits were used and expected results were obtained. I think from all the adhesion studies done, it could be concluded that initial adhesion on pncSi is as good as any other favorable substrate. Hence, the initial low density on pncSi was due…
One of the many contributions Alec S. made to our group is to help us appreciate the importance of the apicobasal (A/B) polarity of endothelial cells. The effects of A/B polarity were demonstrated in his paper: Integrative Biology (2020), 12(11),275–289 where he showed that stimulation of ECs with TNFα from the abluminal chamber caused an…
I wanted to update everyone on the recent attempts at the formation of the a closed flow system by bonding PDMS to our membrane chips, using capillary tubing for the inlet and outlets. Last week when we tried to preform the UV Ozone bonding it was unsuccessful. I initially thought the bonding did not occur…
One of the points I’d like to discuss in my co-culture paper is the advantageous imaging qualities of pnc-Si compared to ‘translucent’ commercial transwell membranes. This has been evident in many of my posts of Live/Dead stained cells, even at 20X. I thought a higher resolution (100X) image would emphasize this point even more since…
Summary My analysis of npMgF2 has relied heavily on both tomogram and segmentation processes. The tomogram generation has thus far been sufficient, giving enough detail to our eye to identify different structures, but turning the tomograms into 3d models has been a lot more labor intensive. Using a neural network classifier (Weka, out of a…
Thanks to SiMPore’s push for TEM grids, we’ve produced a lot of amorphous material in the last month under different deposition conditions. Below I’ve illustrated the different film structures resulting from depositing with and without substrate bias and heating. Without bias, the amorphous film exhibits clusters of thicker/denser? material (darker spots). Intuitively, this makes sense…
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I know it’s a lot more work, but I think we really need separation data at each of these conditions. If the separation efficiency and characteristics change for molecules of a given size, we can speculate that pore deformation occurs at higher pressures that would also increase flow.
Are all these observations reversible? In other words, after spinning at 190psi, can the same device be spun at 1.35psi and have the same results??
Thanks!
I think the data is very noisy (see this figure).
This means that either the measurement isn’t very precise or the material is variable. Lets focus on a single device and get repeat data. My vote is for the 100K microcon.
We may also want to change the way in which we make the measurements. Right now you are using a reverse pipette technique but weighing the water collected could be more accurate?