Process Flow Update

Over the weekend, I resubmitted our adult Adipose-Derived Stem Cell (ADSC) paper titled: Membranes Promote Endothelial Differentiation of Adipose-Derived Stem Cells and Perivascular Interactions There were three main findings: 1.) ADSCs can be induced to express CD31 (PECAM-1) like endothelial cells.  a.) Several prior studies used Vascular Endothelial Growth Factor (VEGF) at 50 ng/mL to…

For a while now I’ve been working on developing and testing the practicality of using nanoporous membranes as filters for highly sensitive assays. Traditional filter-based assays are restricted in their speed by both the time it takes for a particle to find a pore as well as the time it takes for the particle to…

This post presents the data from all of my TEER experiments of BBB co-cultures, with bEnd3 endothelial cells on the bottom and NG101815 glia on the top of PET and pnc-Si transwells.  For all experiments, I used 50000 cells/cm2 as the seeding density.  I always had 3 samples – bEnd3 alone, NG10815 alone and the…

Here is an aggregate of the recent posts/data on the Hemodialysis Project … Device Design/Demo Design idea for assembling multiple chips Gasket design for counter flow dialysis chip Peclet chip demo – > Dialysis Membrane modifications to prevent protein binding (HD PEG) Protein binding is reduced by both Adarza and Shestopolov treatments Damage of pnc-Si by…

While Chris and I were in Boston we deposited our membrane stack on a patterned wafer.  It was annealed at 1000 C and then etched.  Because we didn’t have an accurate deposition rate for Silicon, we overestimated and put down 30 nm (instead of 15 nm).  Still, we can see in the TEM that pores…

Summary:  Annealing to above glass transition T (200 C) doesn’t reduce film stress.  Adjusting post-exposure bake protocol seems to reduce it. A short post to document my efforts at reducing tensile stress in SU-8-3000 coatings by annealing in vacuum to its glass transition temperature (200 °C according to MicroChem).  This is in relation to the…