Lab tours on Friday

I did a quick experiment in order to show everyone what the Tecan allows us to do.  I made a dilution curve of rhodamine 6G in PBS and measured the absorbance and fluorescence with the fluorimeter. I first did an absorbance scan and found that the maximum absorbance was at 530nm (panel A).  Then I…

Introduction Endothelial cell detachment under flow on porous substrates has been a concern in our sepsis research since I began my work in the lab, and some time before that as well. As we plan on moving to microfluidic models that incorporate larger pore substrates and physiological shear stresses for extended periods of time, it…

I’ve been trying for some time to make chips that can dynamically change their surface charge. To this end I’ve made the following NFT chips, which have 12 nm of silver coated in 10 nm alumina:   Note the Aluminum foil coating the insides of the gel box. The silver layer is either photoactive or…

Inspired by JP, we decided to try to etch amorphous (unannealed) wafers this week to see if pinhole density changed.  To my surprise, the amorphous membranes had no pinholes!  Furthermore, I tried to anneal the membranes after the etch and they went flat indicating crystallization!  Refer to the table below for the results. Wafer 565:…

Attached is a series of images we took of two populations of neutrophils above and below the membrane.I’m including by methodology for those who are interested:One population was pre-labeled with AF488 (green) antibody against L-selectin, the other with AF546 (red). The green population was suspended in a low protein buffer (~1% serum) and a 20…