EO w/pnc-Si at Louisville

First, a quick publication update. Recently we’ve published a paper on our efforts towards computationally analyzing polymorphonuclear leukocyte (PMN) trafficking on a vascular endothelium via hand-built µSiM flow cell devices. We record videos in phase contrast with a 40x objective and are able to delineate PMN state, speed, and persistence by leveraging ML algorithms and…

A droplet (3uL) of 0.117 uM actin solution was added on membrane W303(0,2). Let the membrane sit in humidity Chamber for 20 min. Remove excess liquid. Add equal volume of Uranyl Acetate on membrane. Wait 90 sec. Remove excess liquid. Image was taken right after sample preparation. Xiao

Previously, we showed that our method of pll-peg coating is effective for creating non-fouling substrate which can prevent protein adsorbtion, and this method can be used to create a non-fouling pattern on the surface. In the next step, we wanted to make sure that whether our peg coating is effective for preventing cell adhesion or…

Introduction Much of the background information for this set of experiments can be found in my previous post. In short, endothelial cells mainly express two receptors to the sphingolipid S1P, S1PR1 and S1PR3. S1PR1 is characterized as a barrier enhancing signaler, while S1PR3 appears to elevate vascular permeability while promoting leukocyte capture. Based on TEER…