The role of tRNAs on Cell Phenotypes

Transendothelial electrical resistance measurements are expressed in the units . Where the measured resistance is multiplied by the area of the membrane to enable a comparison between measurements in different systems. We have often wondered why, but the reasoning for this normalization is straightforward. Consider the simplified ‘transwell’ geometry where a membrane separates two compartments…

Attached is a series of images we took of two populations of neutrophils above and below the membrane.I’m including by methodology for those who are interested:One population was pre-labeled with AF488 (green) antibody against L-selectin, the other with AF546 (red). The green population was suspended in a low protein buffer (~1% serum) and a 20…

Here are the results for air permeability testing of W501. The sample closest to the origin had one very small (very difficult to see on Brian’s scope unless viewing at 100x) pinhole, the middle sample had 3 very small pinholes. The completely intact sample had a permeability of .773 cc/(cm^2*atm*s). It seems that the pinholes…

The blog has migrated to a new hosting provider. This means that the passwords for owncloud and the blog are no longer in sync. Owncloud has been upgraded from 5.0.18 to 7.0.4. The passwords have not been changed and are the same used for the Wikis, which now reside on their own domain. Any issue,…

Goal In preparation for experimentation with hCMEC/D3, an immortalized BBB cells line, I characterized barrier formation by taking TEER. I want to find peak barrier formation to determine what day to run assays. Methods hCMEC/D3 were maintained and passaged in EGM-2 (EBM-2 + multiple gfs + gentamicin + 2.5% FBS) with growth factor concentrations matching…