The blog has migrated to a new hosting provider. This means that the passwords for owncloud and the blog are no longer in sync.
Owncloud has been upgraded from 5.0.18 to 7.0.4. The passwords have not been changed and are the same used for the Wikis, which now reside on their own domain.
Any issue, do let me know.
Overview: Bob etched 4 non-porous membranes, on the backside of the membrane. HUVECs were to be grown on these etched non-porous SiO2 membranes and compared to unetched non-porous membranes. Below Bob describes how the membranes were etched: “Four of the non-porous SiO2 membranes were placed on a ø6” carrier wafer with the membrane facing up in the…
Over the last few months, I have been trying to reestablish my SERS measurements, and generate a paper based on SERS sensing with our nanomembranes. Previously, I have created magnesium fluoride coated substrates that seem to have better SERS performance than just NPN alone. Combining our TEM imaging with SERS data would be a powerful…
Our first attempt to “reverse” centrifuge materials using our standard 5-slot filters made of NPN from wafer 1070 are detailed in a previous post using GEN2 SEPCON assemblies. In this post we modify our method to more closely match the Sartorius Centrisart approach. To that end, we switch to using GEN3 SEPCONs because they are easier…
I used the transwell track etched membranes for these permeability experiments. I first looked at 5nm gold in 10mM KCl. After 24 hours the retentate and filtrate had just about equilibrated: We can compare these scans to the stock 5nm stock solution and see that the height of the 525nm peak is related to the…
A bit of background: In order to quantify how permeable a tissue barrier is, we use a method known as trans epithelial electrical resistance (TEER). Cells are grown on a permeable growth substrate in a transwell format. When the cells are not confluent, there is a continuous diffusion of ions across the two sides of…
Last week, JP deposited 25nm SiO2 for me on wafer 644 (which I cleaned in the UV/Ozone before I gave it to him). Unfortunately, someone broke the E-Beam that day and JP was only able to deposit on 1 side of the wafer (the solid side). I did some experiments on the wafer anyway. First,…