Cellulose C-Dot separations

I used 100kD Millipore Celllulose membranes to separate the 2 C-dot particles.  The results are similar to those I’ve obtained with pnc-Si; the 10nm particles pass while the 30nm ones do not.

Cy5 10nm particles:

picture-10

Sulforhodamine 30nm particles:

picture-11

Here are the sieving ratios:

Particle Ratio
10nm (abs) .60
10nm (fluor) .59
30nm (abs) .02
30nm (fluor) .01

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