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Protein Binding by Suspended Graphene Oxide
Experiments were conducted to determine how effectively protein is bound by suspended graphene oxide (GO) particles. This is important for our application because we hope to use GO as a membrane to bind toxins present in blood. BSA (bovine serum albumin) was used as the test protein. Test samples consisted of a mixture of BSA…
Initial silicon film density affects porosity
For the last three weeks, we have increased the substrate bias power from 25 W to 32 W (for both oxide and a-Si) to adress some film quality issues. I have found that it is “harder” to open pores in a film deposited at higher bias. Below are micrographs showing the difference in porosity for…
Single-Well CytoVus w/ SiO2 Membrane – Comparisons
I’ve run a few comparative studies with the CytoVu devices that have SiO2 membranes in them. From a previous NRG discussion, here is the comparison of a single location with only the fluorescent images, and that same location but with phase included: In the phase image, the pores can be seen through the cells, especially evident in…
(Lack of) Effects of Anti-CD29 antibody on endothelial barrier function
In the past, I have been working on developing mechanisms to inhibit neutrophil migration through endothelial basement membrane. Please find the previous blogs on the following links https://trace-bmps.org/updates-on-neutrophil-collagen-migration-experiments/ https://trace-bmps.org/manipulating-neutrophil-migration-through-the-vascular-barriers/ https://trace-bmps.org/sem-imaging-of-neutrophil-transmigration-through-collagen-gel/ This is a small update on the following. CD29 is beta-1 integrins, which are used by neutrophils and endothelial cells to adhere to basement…
Peristaltic pump volumetric flow rate experiment data & Calculations
Just wanted to drop this data here in case it may be useful for others in the lab. I calculated the volumetric flow rates for one of the peristaltic pumps used in the lab for the flow circuit in cell-culture. The setting # is the bar # on the peristaltic pump controller (the 10 bars…
Highly porous Parylene membranes with precise control over pore size using a single mask
Parylene-C is a biocompatible, inert, FDA-approved and nondegradable material, which can be used to fabricate mechanically robust thin membranes. Parylene is used in the medical device industry for coating implantable devices and in the microelectronics industry as insulator. Furthermore, it has been used in biomedical research for a wide variety of applications. In addition, parylene-C…