Jim’s Talk at Spring Brain Conference in Sedona, AZ

Building on my recent posts I ran an experiment with SC267 testing the ability of BSA and DDM to diffuse through UV-Ozone treated (20min at 150C) and untreated samples. I used four samples testing both BSA and DDM in the treated and untreated cases using Jess’ diffusion cell. Again 6uL was placed on the membranes…

160112 LO Grant Update

Last week Dave deposited sep-cons ( cell culture mask) with 3 different thickness namely the regular 15nm , 30 nm and 50nm ( SC 142, 143,144). These wafers were characterized and it was noted that the 15 nm had a high pinhole density and the 30 and 50 nm films were low pinhole density.  The…

The Tecan Nanoquant plate is amazing.  For those of you who are unaware, we were able to demo a quartz plate that enabled absorbance measurements with only 2ul drops.  It took a little bit for me to figure it out, but it really is a great tool for this kind of study. The ultra low…

The new smaller EQ setup that Tom came up with were tested in these experiments. The benefit is that there is much less dilution of our filtrates. 500ul of our sample (in these cases we tested 1.0 mg/mL and 0.5 mg/mL brain extract) is loaded into the SepCon and 250uL of buffer fills the reservoir…

Kevin Healy is a Phase I and Phase II awardee of the NIH Tissue Chip initiative. The goal of this initiative is to create ‘mini-organs’ ‘organs-on-a-chip’ ’tissue chips’ or ‘microphysiological systems’ (MPS) for higher throughput and more reliable drug discovery. The effort is also motivated by the failure of recent animal models to predict drug…