Similar Posts
Simple bioreactor with the square, 5-slit pnc-Si chip
ByHenryHi all! Above is an image of the bioreactor made from the square, 5-slit pnc-Si chip and a PDMS structure. Fused-silica capillary tubings were inserted into the bioreactor to form the inlet and the outlet for the transfer of the cells into the lower compartment, which is made entirely out of PDMS. Note that the…
A Practical Guide to Membrane Transfer
We’ve made a few academic inquiries so far into the theory which governs our nanoporous membrane transfer, but engineers as we are, the pace of our practical knowledge has outstripped that of our theoretical understanding. Here, I want to share a summary of what we know about these transferred membranes from a practical perspective, oriented…
Concurrent TEER and Phase Microscopy with Yuchen Wang
For the first time, we did time-lapse phase microscopy (1 frame / 5 seconds) looking at endothelial cells through the ITO in the static BBB device (device A bottom left well) and measuring the TEER concurrently. Here’s the video, just shy of 15 minutes of recording. (reporting a low resistance: 5-10 ohms, shorted). TEER Test…
Fibroblast wound healing with small EVs
Wound healing experiments I have been isolating extracellular vesicles (EVs) from adipose-derived stem cells (ADSCs) and using them in wound healing experiments. For isolation, I follow the same protocol as outlined here. For these experiments, I used small EVs, which are collected in the last step of the isolation process. I began by seeding human…
SU-8 Surface Modification
Last summer, I attempted to modify the surface of SU-8. The purpose of the experiment was to covalently bond poly(ethylene glycol) (PEG) to SU-8, which would be useful when the SU-8 is used as a scaffold on the membranes. The method of surface modification was derived from the attached article, which can be found here. …
Effect of increasing dialysate flow rate on diffusive mass transfer of urea, phosphate and β2-microglobulin during clinical haemodialysis
ByJimBhimani(Ward)10NDT This is a useful paper for our work on Hemodialysis. The authors examine the clearance rates of urea, B2 microglobulin and phosphate as a function of dialysate flow rate . Two things surprise me in this paper: 1) the clearance levels achieved are much higher than I’ve come to expect, and 2) the clearance…