Wound healing experiments I have been isolating extracellular vesicles (EVs) from adipose-derived stem cells (ADSCs) and using them in wound healing experiments. For isolation, I follow the same protocol as outlined here. For these experiments, I used small EVs, which are collected in the last step of the isolation process. I began by seeding human…
Last time this project was discussed at NRG, Henry was the project lead (we miss you, Henry). He was observing bladder epithelial cell (BdEC) invasion and migration in co-culture with T24 bladder cancer cells. Here are links to his previous posts. https://trace-bmps.org/bdec-t24-co-culture-wound-healing/ https://trace-bmps.org/bdec-t24-co-culture-invasion-assay-preliminary/ We have transitioned away from the invasion assay and moved towards transwell…
A few weeks ago, Henry and I ran a migration/wound healing experiment using two cancer cell types: T24 (bladder cancer) and MDA-MB-231 (breast cancer). MDA cells are highly migratory, while T24 cells are less so. We wanted to test if the exosomes from MDA cells would cause T24 cells to become more migratory. This was…
Experiments were conducted to determine how effectively protein is bound by suspended graphene oxide (GO) particles. This is important for our application because we hope to use GO as a membrane to bind toxins present in blood. BSA (bovine serum albumin) was used as the test protein. Test samples consisted of a mixture of BSA…
Recently we were interested in finding out how many particles from our immobilized graphene oxide (GO) nanolaminates are delaminated into suspension due to shear stress. We wanted to compare this concentration to those found in our previous hemolysis studies to determine if the delaminated particles would lyse red blood cells. In order to determine this,…
For this post, Henry and I collected data for hemolysis and NTA sizing of soluble graphene oxide (GO). Hemolysis– prior work on the hemolysis testing of immobilized GO The purpose of the hemolysis experiment was to confirm that immobilized GO is the preferred from of GO for use in a portable hemodialysis machine. It…