R6G diffusion observed through the plastic substrate

I finished 3 independent experiments of air-blood barrier co-cultures – these consist of bEnd3 endothelial cells on 1 side and RLE-6TN lung epithelial cells on the opposite side of the membranes.  We didn’t expect these cell types to potentiate TEER in anyway (like we expected glial cells to tighten endothelial barrier function).  However, our collaborators…

Last week Dave deposited sep-cons ( cell culture mask) with 3 different thickness namely the regular 15nm , 30 nm and 50nm ( SC 142, 143,144). These wafers were characterized and it was noted that the 15 nm had a high pinhole density and the 30 and 50 nm films were low pinhole density.  The…

I’ve run a few comparative studies with the CytoVu devices that have SiO2 membranes in them.  From a previous NRG discussion, here is the comparison of a single location with only the fluorescent images, and that same location but with phase included: In the phase image, the pores can be seen through the cells, especially evident in…

20nm polystrene beads in 1x PBS passed through w218. This wafer looks a little strange, like it was not fully etched (Dave do you know what happened to this one?). This was just a first stab at the benchtop diffusion with PS, so I didn’t use a “good” membrane. At any rate, the PS went…

Roundness vs. Equivalent Diameter plots for five eccentric wafers: There really doesn’t seem to be a correlation between equivalent diameter and roundness. However, there does appear to be a rough downward trend in SC 016 and SC 258. Mean Roundness vs. Wafer Number graph with error bars included: Without the error bars, it looked like…

This post is meant to conclude the work that I have completed this summer with the double slot single pass devices; recently I have conducted additional experiments and collected ultrafiltration data. In my previous post, “Single Slot Inverted-Chip and Double Slot: Single-Pass Dialysis Devices” https://trace-bmps.org/single-slot-inverted-chip-and-double-slot-single-pass-dialysis-devices/ I was exploring how various analyte / dialysate flow rate…