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Paper Summary – Surface Engineered Porous Silicon for Stable, High Performance Electrochemical Super Capacitors
Pint Silicon Supercapacitor Tejas sent me an interesting article about Silicon-based supercapacitors. I have made a previous post on potential uses for our membranes as separators in EDLC (electrical double layer capacitors), and one of the problems I posited was that the electrolyte chemistry seemed particularly inimical to silicon (KOH, Na2SO4). The authors in…
Attempts at Co-culture
Attached is a series of images we took of two populations of neutrophils above and below the membrane.I’m including by methodology for those who are interested:One population was pre-labeled with AF488 (green) antibody against L-selectin, the other with AF546 (red). The green population was suspended in a low protein buffer (~1% serum) and a 20…
Disposable PDMS diaphragm pumps
In order to include pumps on the prototype blood analyzer cartridges, it was necessary to implement a small pump using the existing knife-cut PDMS layer layup technique. The diaphragm architecture was selected due to ease of operation and minimal additional part requirements. CAD model of pump in Autodesk Inventor Bottom view of fabricated pump. The…
Single-Well CytoVus w/ SiO2 Membrane – Comparisons
I’ve run a few comparative studies with the CytoVu devices that have SiO2 membranes in them. From a previous NRG discussion, here is the comparison of a single location with only the fluorescent images, and that same location but with phase included: In the phase image, the pores can be seen through the cells, especially evident in…
STTR: Silicon Nanomembranes for Ab-labeled NP clean-up
ByJimSubmitted on April 7, 2014. Posted to NRG Cloud under NRG/Funding. Figure 2. Dead End Filtration with SEPCONTM (A) SiMPore’s SEPCONTM spin cup with a silicon nanomembrane chip. B) Dead end filtration in a centrifuge. The direction of the centrifugal force is indicated with the gravity vector g. Figure 3. Nanoporous Silicon Nitride (A) pnc-Si…
PDMS-filled wells don't increase sensitivity for HMM detection
This post is related to the HIV drug screening project that Carrie has been working on. To refresh everyone’s memory, the aim of this project is to use pnc-Si as a filter to separate high molecular mass (HMM) protein-RNA complexes from smaller protein monomers/multimers. Small molecules that break down the HMM form of this protein…