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Au separations with SiN-NPs
Ultimately we want to use SiN-NP filters to separate viruses or some other biological species, but repeated attempts to pass 20 nm fluorospheres in water or PBS have failed. Au particles, however, have passed through the filters. This post reports the results of Sarah and I attempting to pass Au particles from 20 to 100…
Streaming potential in narrow pores
Narrow pore EO theory In narrow pores that have a significant Debye length, we need to use the same corrections on surface potential in streaming potential theory that we used in electroosmosis theory (click on link above for EO theory). In essence, the equation for streaming potential (E) becomes: where , P is the applied…
Hydraulic permeability in the pressure cell
I’ve recently been exploring hydraulic permeability using the pressure cell. As you may recall, the majority of our permeability measurements have been done in the centrifuge (EQ format or closed bucket). The pressure cell allows for a more accurate measurement of permeability because it is not affected by changing water levels or variable driving pressures….
FLAG Diffusion Experiment Progress Update
For context, see my previous post. Early on in the process of designing an experiment to confirm the usefulness of a CytoVu assembly for use as an assay, it was decided that the best solution for measuring the extremely low concentrations [ng/mL scale] of bacterial alkaline phosphatase (“bAP”) in the filtrate which would indicate what…
Excitation and Emission Wavelengths for Common Fluorophores
Here is the excitation and emission wavelengths for two common fluorophores for transport: rhodamine and fluoroscein. rhodamine fluoroscein max ABS 480 nm 470 nm emission 558 nm 516 nm excitation 524 nm 492 nm The protocol for finding excitation and emission wavelengths of a fluorophore with use of the Tecan can be found here. During…
Tendon-on-Chip Platform for Modeling Inflammation and Fibrosis in Tendon Injury
This is my draft presentation for my CMSR presentation on December 4th. Link