Polymer Presentation

The mechanism of organic dye photobleaching is not well understood, but there are a few methods out there for reducing the rate of photobleaching.  Some literature suggests that photobleaching is due to excess oxygen in solution and the interaction between the dye and oxygen radicals.  Actin research swears by the following “anti-bleaching buffer” to limit…

An initial experiment for membrane deflection characterization using an optical profiler (Veeco Wyco) has been performed. Below are some of the images taken with the tool: (a image was taken at each 0.5 psi interval): Once we get a pressure regulator we will be able to generate numerical data of membrane strength/deflection down to 5.0…

I ran some more tests on the microcon and nanosep filtration systems at 21.36psi (4000RPM) and 194.65psi (12,000RPM). Both are rated for forces up to 14,000 time gravity which is about 340psi but after talking to Tom we thought it best to run it at a slightly lower pressure to account for any possible error…

We’ve been wondering for some time just how the thinness of our membrane will affect the concentration polarization behavior of solutes. Because solutes with sieving coefficients less than 1 are (by definition) moving slower than the solvent around them, in the area before the membrane their concentration increases. In the case of proteins, this so-called…

In order to validate the salt diffusion test I just posted, I’ve been measuring H2O2 diffusion with SC348 and the Amplex Red Assay from Invitrogen.  I first showed that H2O2 (=34 Da) passed SC348 in this experiment.  Here, I just repeated with slightly different standard curves and positive (‘regular’ Sepcon, SC195 = 4% and 13.9nm)…

Introduction These results are thanks to assistance from Sam Walker and his previous posts regarding urea clearance that helped lay the foundation of this experiment. “Single Slot Inverted-Chip and Double Slot: Single-Pass Dialysis Devices” (Walker 2018) https://trace-bmps.org/single-slot-inverted-chip-and-double-slot-single-pass-dialysis-devices/   More information on the Liftoff membranes can be found in the article cited below: “Lift-off of large-scale…