A droplet (3uL) of 0.117 uM actin solution was added on membrane W303(0,2). Let the membrane sit in humidity Chamber for 20 min. Remove excess liquid. Add equal volume of Uranyl Acetate on membrane. Wait 90 sec. Remove excess liquid. Image was taken right after sample preparation.
Xiao
Last week the rotation stage broke in the middle of a deposition. The wafer came out looking very non-uniform, and judging by the color of the film there was at least a 10 nm gradient. Since I’m not sure when the motor stopped, it’s hard to say if the underlying oxide is non-uniform as well,…
Submitted on April 7, 2014. Posted to NRG Cloud under NRG/Funding. Figure 2. Dead End Filtration with SEPCONTM (A) SiMPore’s SEPCONTM spin cup with a silicon nanomembrane chip. B) Dead end filtration in a centrifuge. The direction of the centrifugal force is indicated with the gravity vector g. Figure 3. Nanoporous Silicon Nitride (A) pnc-Si…
In the last month, we’ve run several sets of 15 nm pnc-Si wafers over a range of RTP temperatures. Below is a chart that illustrates the current pore size control we have by tuning annealing temp. The 900 C, 950 C, and 1000 C samples were from wafers SC 231, SC 314, and SC 316,…
I am working with different electrode materials as a substitute for ITO for my TEER measurement device. I used silver-silver chloride electrode and platinum-iridium ribbon (90:10) for that purpose. I made AgCl electrode by dipping about an inch of silver wire in silver chloride ink and letting it dry for half hour. I obtained the…
Attached is a paper I’d like to review on Thursday (June 11, 2015). This group is using two planar surfaces in an attempt to mimic a 3D cell culture environment. This is similar to our claims that cells growing on a permeable membrane can be akin to a slice of a 3D matrix. I met…
As the device animation shows, the layout of the chip has changed. The incorporation of through-vias in the bulk silicon allows me to eliminate a gasket wrapping around the filter, which was a large source of leaks. I used ozone bonding for 8 minutes for each layer to join the device together. I initially taped…
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There appears to be molecular structure in these images! In my experience people need to label with larger actin binding proteins to see this type of structure. I know Karen is doing some comparisons on carbon grids.
Could be good.
Here is a useful web page on electron microscopy that uses actin filaments as a reference molecule. The good news is that I think our images show hints of the ‘rope-like’ actin structure you can see in Figure 1.8.
http://www.mih.unibas.ch/Booklet/Lecture/Chapter1/Chapter1.html