A droplet (3uL) of 0.117 uM actin solution was added on membrane W303(0,2). Let the membrane sit in humidity Chamber for 20 min. Remove excess liquid. Add equal volume of Uranyl Acetate on membrane. Wait 90 sec. Remove excess liquid. Image was taken right after sample preparation.
Xiao
As Aim 1 of my thesis involves using theoretical models to be able to predict particle capture, it is important for me to fully understand how each model system behaves when compared to experimental results. One of the most important of these systems is the pressure drop across the membrane in our devices. I have…
I’ve been working with the Corning transwell inserts and our SepCon units a bit recently. The addition of the Dremel last week has allowed better control while cutting the transwells, even without the right angle attachment (which will allow cutting in the “drill press” mode). I attached a picture of the process and final unit….
In prep for manuscript writing I came across a paper that says they’ve created a super efficient EO pump that uses extremely low voltages. Their top flow rate (.125 mL/(min V cm2) ) is comparable to the flow rates I see with our materials (144 mL/(min V cm2)). They run their experiments at 3 V…
Here is a slightly enhanced version of Mike’s latest hydraulic permeability data for commercial devices with PES membranes. The data at 22 and/or 34 PSI are a bit strange. Still the long-term trend seems downward. Similar data for PNC-Si membranes should show no pressure dependence. We need to get a version of this figure that…
Adding pockets (250 micron long, 76 micron deep) to the bottom of the channel (channel is 500 microns wide, 125 deep) in a 4 layer PETL (1 inch long chip) promotes disruption of laminar flow.
Intro Understanding how the Au film coats the inner part of the npMgF2 is essential to understanding how the photonic properties of the npAu/npMgF2 sensor arise. From SEM, we are able to resolve little cones of Au, but unable to see how rough/homogeneous/open the Au layer is. Our hypothesis is that these little cones of…
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There appears to be molecular structure in these images! In my experience people need to label with larger actin binding proteins to see this type of structure. I know Karen is doing some comparisons on carbon grids.
Could be good.
Here is a useful web page on electron microscopy that uses actin filaments as a reference molecule. The good news is that I think our images show hints of the ‘rope-like’ actin structure you can see in Figure 1.8.
http://www.mih.unibas.ch/Booklet/Lecture/Chapter1/Chapter1.html