A droplet (3uL) of 0.117 uM actin solution was added on membrane W303(0,2). Let the membrane sit in humidity Chamber for 20 min. Remove excess liquid. Add equal volume of Uranyl Acetate on membrane. Wait 90 sec. Remove excess liquid. Image was taken right after sample preparation.
Xiao
While serving on an NSF MRI (Major Research Instrumentation program) review panel today the director shared his analysis of funding probabilities as a function of score. The analysis was only done for the Directorate of Math and Physical Sciences, but I bet it is a good rule of thumb throughout NSF. I sketched his power…
I know this is my third post today, but this is not a data post. It’s more of a PSA. If you want to add beautiful equations into your post (like I did here) then rather than generating a bunch of images and inserting them into the post, you can simply use . is the…
I attended the 2014 Gordon Research Conference (GRC) on Fuel Cells in August. For those of you not familiar with Gordon conferences, I will make a plug for them as a great venue for learning much detail and interacting closely with many of the major contributors in a specific field. GRC has a wide variety…
Motiviation and Background: Nakul and I have been performing a lot of DNA filtration and attempted DNA shearing experiments. We found that large DNA fragments (>1000 and >2000 bp) pass through ~30-40 nm max pore size membranes when pressurized to 10 psi (even in low salts). This is in contrast to the Bernhard data where…
In these discoloration tests, I looked at the components of HBSS. HBSS consists of NaCl, KCl, 0.048 mg/mL Na2HPO4, 0.06 mg/mL KH2PO4, glucose and 0.35 mg/mL NaHCO3. In a previous post, I showed that NaCl, KCl and glucose didn’t cause discoloration (previous post). So, I made HBSS from scratch and I combined the various phosphate…
This initial aim of this project was to obtain “pockets” with sub-100nm pores, while the top opening is much larger. Dr. Carter is the mastermind behind this project. The following simple fabrication process diagram explains the current approach. After initial failed experiments for obtaining very small openings, Marcela and I aimed for larger bead sizes…
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There appears to be molecular structure in these images! In my experience people need to label with larger actin binding proteins to see this type of structure. I know Karen is doing some comparisons on carbon grids.
Could be good.
Here is a useful web page on electron microscopy that uses actin filaments as a reference molecule. The good news is that I think our images show hints of the ‘rope-like’ actin structure you can see in Figure 1.8.
http://www.mih.unibas.ch/Booklet/Lecture/Chapter1/Chapter1.html