memBrains

R61/R33 Update to NIH on Nov. 9, 2022

ByJim November 15, 2022

Attached is a PDF presented to the NIH at our annual update meeting that included other funded project teams.

R61_R33HL154249 11-22 Update (Low Res)

memBrains

Assessing the Permeability of Two Barriers in the Post-capillary Neurovascular Unit Part 2: Measuring R2
ByJim May 5, 2024May 6, 2024
In Part 1 we explained that the composite barrier in our model of the post-capillary NVU is determined by RBBB = RBlB + R2 + RBrB where is R2 is the resistance of the ‘perivascular space’ in our chip. Unlike the other two, the perivascular space is not a regulated cellular barrier that we need…
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memBrains | NRG | μSiM

Collagen IV deposition by hCMEC/D3 (endothelial cell) and HBVP (pericyte) co-cultures in the μSiM-hNVU
ByLouis Widom February 24, 2021February 25, 2021
Note: A postscript regarding a gelatin assay to determine MMP-9 activity was added to this post on Feb 25, 2021. Introduction The blood-brain barrier (BBB) plays an important role in maintaining brain chemistry by restricting the permeability of undesirable small molecules and cells. Inflammatory diseases may disrupt the BBB and promote the development of neurological…
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memBrains

QC on Dual Scale Membranes (Lot 1383)
ByDan Ahmad December 15, 2020December 15, 2020
Recently we have received dual scale (nanoporous and microporous) membranes from SiMPore at two porosities: 0.625% and 1.25%. These feature 3 µm circular pores (Lot 1383). We routinely perform QC checks on membrane deliveries, so the following is report on this delivery. First, I’d like to note that we were supposed to receive equal amounts…
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memBrains | NRG

Optimizing Cytomix Stimulation on Mono- and Coculture BBB Models
ByMolly McCloskey June 9, 2022March 5, 2024
Introduction The µSiM BBB model is being developed at UR with the goal to understand the impact of sepsis on the brain. Here, we use an EECM-BMEC-like cell (iSPC-derived brain endothelial cells) [1] monoculture model and an EECM-BMEC-like cell and brain pericyte-like cell (BPLC; iSPC-derived brain pericytes) [2] coculture model to optimize the concentration of…
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memBrains | NRG | μSiM

Gene expression analysis of HUVECs cultured on uSiM (static vs flow)
ByMehran Mansouri October 20, 2022November 17, 2022
Introduction Studies have shown that the shear-stimulation of endothelial cells results in the upregulation of the shear-sensitive transcription factor Kruppel-like factor 2 (KLF2). KLF2 plays a critical role in the regulation of endothelial activity such as the regulation of vascular tone, anti-inflammatory responses, and antithrombotic functions. KLF2 is also responsible for the upregulation of endothelial…
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memBrains | NRG

Little Back Dots in Coated Controls … How We Wonder What You Are.
ByMichelle Trempel March 10, 2022June 8, 2022
Introduction We are investigating some issues seen with long term attachment (up to 6 days) of the EECM-BMEC-like cells seen by collaborators in Bern, where they noticed black aggregates on the coated control devices that get worse at day 6. Some attachment issues and aggregates have also been seen at the University of Rochester in…
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