R61/R33 Update to NIH on Nov. 9, 2022
Attached is a PDF presented to the NIH at our annual update meeting that included other funded project teams.
Attached is a PDF presented to the NIH at our annual update meeting that included other funded project teams.
Introduction Mural cells, including smooth muscle cells and pericytes, are important for supporting endothelial cells in the vasculature [1]. They play a role in angiogenesis, and contribute to the development of the vascular basement membrane that provides structure for blood vessels [1,2]. Numerous in vitro models of the blood-brain barrier (BBB) have incorporated pericytes alongside…
AlineFlowBottomChannelSup AlineFlowBottomChannel
Introduction The capability of the m-μSiM for establishing an hiPSC-derived blood-brain barrier (BBB) in bioengineering and bioscience laboratories has been shown previously [1]. To further demonstrate interlaboratory agreement and successful dissemination of components and protocols, m-μSiM was used at RIT (Abhyankar lab) to establish an hiPSC-BMECs monolayer. Method All reagents and steps were used based…
Introduction In the Glading Lab we study a rare disease called Cerebral Cavernous Malformations (CCMs). In this disease, brain capillaries develop greatly enlarged lumens and a disrupted blood-brain barrier. A genetic cause of the disease is the presence of a mutation in KRIT1 that causes loss of KRIT1 protein function in the cells. KRIT1 binds…
20210122.flow-movie-demo I attached a link to a short presentation regarding the flow movie setups. The figures and videos will be explained in detail during the meeting on Friday.
Slide deck from Large Group Meeting 12-21-12 (PPT) Access requirements for the AFM cantilver to contact the cells requires the cells to be grown on the membrane surface in what is typically the bottom channel of A-line devices (Typical A-line configuration is shown in slide 2). A PDMS block is attached to the well side…