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Beautiful images of aligned cells on the backside of the device! Did you take any images before flow so we can make the claim these are aligned? You mentioned that some cells didn’t survive? Can you explain where?
I might have an image of these cells before flow but it is through the clamp system.I’ll make sure to get those before and after shots when I run this for the AFM measurements. I’ll add to this post next week. The cells that are in the channel of the aline are undergoing much higher shear stresses and the cells get strip off. I noticed that the entrance side seemed to retain more cells then the exit side but maybe it is just me. The membrane is 100% confluent and healthy and I can run two Alines with same system!